Production of Liposome from Sphingomyelin by Ultrasonic Device under Supercritical Carbon Dioxide
Sphingomyelin is a type of sphingolipid found in animal cell membranes and can be exploited as an amphiphilic liposome component in the manufacture of liposomes capsule. Here, the fabrication of liposomes from sphingomyelin suspension solution via ultrasonicâ€“supercritical carbon dioxide (CO2) was studied. The experiments were conducted at temperatures of 40 â€“ 60 oC and pressures of 10 â€“ 20 MPa in batch process. As a starting material, sphingomyelin powder was dissolved and dispersed in distilled water. The TEM images indicated that the liposomes products were successfully formed in spherical and sphericalâ€“like shape morphologies with bimodal size at 91 â€“ 220 nm and 396 â€“ 955 nm. The liposomes products with smaller diameter were obtained when the experiments were conducted at higher operating pressure. The DLS measurement showed that the size distribution of liposomes products was increased with increasing operating temperature due to the aggregation. Based on the result, this process seems a powerful technique for organic solvent free liposome production technology from sphingomyelin solution for industrial purposes.
Daraee, H., Etemadi, A., Kouhi, M., Alimirzalu, S., Akbarzadeh, A., â€œApplication of liposomes in medicine and drug deliveryâ€, Artif. Cells Nanomed. Biotechnol., vol. 44, no. 1, pp. 381â€“391, 2016.
Pattni, BS., Chupin, VV., Torchilin, VP., â€œNew developments in liposomal drug deliveryâ€, Chem. Rev., vol. 115, no. 19, pp. 10938â€“10966, 2015.
Alavi, M., Karimi, N., Safaei, M., â€œApplication of Various Types of Liposomes in Drug Delivery Systemsâ€, Adv. Pharm. Bull., vol. 7, no. 1, pp. 3â€“9, 2017.
Gomezâ€“Hens, A., Fernandezâ€“Romero, JM., â€œAnalytical methods for the control of liposomal delivery systemsâ€, Trends Anal. Chem., vol. 25, no. 2., pp. 167â€“178, 2006.
Mozafari, MR., Johnson, C., Hatziantoniou, S., Demetzos, C., â€œNanoliposomes and their applications in food nanotechnologyâ€, J. Liposome Res., vol. 18, no. 4, pp. 309â€“327, 2008.
Patil, YP., Jadhav, S., â€œNovel methods for liposome preparationâ€, Chem. Phys. Lipids, vol. 177, pp. 8â€“18, 2014.
Kon, T., Goto, M., â€œPreparation method of liposomes and multilamellar liposomeâ€, Japan Patent 2016-077165.
Herrero, M., Mendiola, JA., Cifuentes, A., Ibanez, E., â€œSupercritical fluid extraction: Recent advances and applicationsâ€, . J. Chromatogr. A, vol. 1217, no. 16, pp. 2495â€“2511, 2010.
Goto, M., Kanda, H., Wahyudiono, Machmudah, S., â€œExtraction of carotenoids and lipids from algae by supercritical CO2 and subcritical dimethyl etherâ€, J. Supercrit. Fluids, vol. 96, pp. 245â€“251, 2015.
Otake, K., Shimomura, T., Goto, T., Imura, T., Furuya, T., Yoda, S., Takebayashi, Y., Sakai, H., Abe, M., â€œPreparation of liposomes using an improved supercritical reverse phase evaporation methodâ€, Langmuir, vol. 22, no. 6, pp. 2543â€“2550, 2006.
Kadimi, US., Balasubramanian, DR., Ganni, UR., Balaraman, M., Govindarajulu, V., â€œIn vitro studies on liposomal amphotericin B obtained by supercritical carbon dioxideâ€“mediated processâ€, Nanomed., vol. 3, no. 4, pp. 273â€“280, 2007.
Meure, LA., Foster, NR., Dehghani, F., â€œConventional and dense gas techniques for the production of liposomes: a reviewâ€, AAPS Pharm. Sci. Tech., vol. 9, pp. 798â€“809, 2008.
Karn, PR., Cho, W., Park, HJ., Park, JS., Hwang, SJ., â€œCharacterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham methodâ€, Int. J. Nanomed., vol. 8, pp. 365â€“377, 2013.
Bhuvana, M., Dharuman, V., â€œTethering of spherical DOTAP liposome gold nanoparticles on cysteamine monolayer for sensitive label free electrochemical detection of DNA and transfectionâ€, Analyst., vol. 139, no. 10, pp. 2467â€“2475, 2014.
Nomura, F., Honda, M., Takeda, S., Inaba, T., Takiguchi, K., Itoh, TJ., Ishijima, A., Umeda, T., Hotani, H., â€œMorphological and topological transformation of membrane vesiclesâ€, J. Biol. Phys., vol. 28, no. 2, pp. 225â€“235, 2002.
Choi, HJ., Song, JM., Bondy, BJ., Compans, RW., Kang, SM., Prausnitz, MR., â€œEffect of osmotic pressure on the stability of whole inactivated influenza vaccine for coating on microneedlesâ€, PLoS one, vol. 10, no. 7, pp. e0134431, 2015.
Hayashi, M., Nishiyama, M., Kazayama, Y., Toyota, T., Harada, Y., Takiguchi, K., â€œReversible morphological control of tubulinâ€“encapsulating giant liposomes by hydrostatic pressureâ€, Langmuir, vol. 32, no. 15, pp. 3794â€“3802, 2016.
Yaghmur, A., De Campo, L., Sagalowicz, L., Leser, ME., Glatter, O., â€œEmulsified microemulsions and oilâ€“containing liquid crystalline phasesâ€, Langmuir, vol. 21, no. 2, pp. 569â€“577, 2005.
Liu, AL., Iglic, A., â€œAdvances in Planar Lipid Bilayers and Liposomes, Volume 10â€, 1st Edition, Academic Press, 2009
Monteiro, N., Martins, A., Reis, RL., Neves, NM., â€œLiposomes in tissue engineering and regenerative medicineâ€, J. R. Soc. Interface, vol. 11, no. 101, pp. 20140459, 2014.
Risselada, HJ., Marrink, SJ., â€œCurvature effects on lipid packing and dynamics in liposomes revealed by coarse grained molecular dynamics simulationsâ€, Phys. Chem. Chem. Phys., vol. 11, no. 12, pp. 2056â€“2067, 2009.
Zook, JM., Vreeland, WN., â€œEffects of temperature, acyl chain length, and flowâ€“rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing deviceâ€, Soft Matter, vol. 6, no. 6, pp. 1352â€“1360, 2010.
Justo, OR., Moraes, AM., â€œAnalysis of process parameters on the characteristics of liposomes prepared by ethanol injection with a view to process scaleâ€“up: effect of temperature and batch volumeâ€, Chem. Eng. Res. Des., vol. 89, no. 6, pp. 785â€“792, 2011.
Torchilin, VP., Omelyanenko, VG., Lukyanov, AN., â€œTemperature dependent aggregation of pHâ€“sensitive phosphatidyl ethanolamineâ€“oleic acidâ€“cholesterol liposomes as measured by fluorescent spectroscopyâ€, Anal. Biochem., vol. 207, no. 1, pp. 109â€“113, 1992.
Toh, MR., Chiu, GN., â€œLiposomes as sterile preparations and limitations of sterilisation techniques in liposomal manufacturingâ€, Asian J. Pharm. Sci., vol. 8, no. 2., pp. 88â€“95, 2013.
Li, J., Wang, X., Zhang, T., Wang, C., Huang, Z., Luo, X., Deng, Y., â€œA review on phospholipids and their main applications in drug delivery systemsâ€, Asian J. Pharm. Sci., vol. 10, no. 2, pp. 81â€“98, 2015.
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