Patogenicity of Bacillus thuringiensis which Isolated from Tidal Ecosystem against Diamond Backmoth Larvae, Plutella xylostella Linn
Keywords:
Pathogenicity, Bacillus thuringiensis, Tidal ecosystem, Plutella xylostellaAbstract
The purpose of this research was 1) the exploration of Bacillus thuringiensis in soil, water, and organic matter contained in the tidal area of the South Kalimantan and Central Kalimantan; 2) Comparing the pathogenicity among isolates of B. thuringiensis were found against Plutella xylostella larvae; 3) To test the effectiveness of the highest pathogenicity B. thuringiensis isolates from isolates of exploration results against P. xylostella larvae. Soil samples taken from six areas of land in tidal area of the Barito Kuala, Banjarmasin, Banjar district, Province South Kalimantan, Pulang Pisau and Kapuas of Central Kalimantan Province. Isolation of bacteria and pathogenicity tests conducted in the the laboratory of Plant Pests and Diseases Department of the Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru. Research carried out for 8 months. Pathogenicity carried out by determining the level of LC50 value from each B. thuringiensis were found by probit analysis. Efficacy Test of B. thuringiensis implemented using completely randomized design, which consists of 5 treatments and 4 replicates ie a) 2 cc suspension of Bacillus thuringiensis/ l water; b) 3 cc suspension of Bacillus thuringiensis/l water; c) 4 cc suspension of Bacillus thuringiensis/ l water; d) Application of klorfluazuron insecticide, the trade name Atabron with a concentration of 2 cc / l water; e) Applied with water. Differences between treatment effect is determined by Duncan's Multiple Range Test (DMRT). The final conclusion is that: a) Results of exploration was found 11 (eleven) isolates of B. thuringiensis in the areas of tidal ecosystems; b) The higher the concentration of cells of B. thuringiensis more larvae of P. xylostella were dead; b) the high pathogenicity of B. thuringiensis isolates obtained on isolated from drains ecosystems (sewers) on forest with LC50 values of 2.41 x 107 cells / ml of water; d) The concentration of B. thuringiensis the most effective in reducing leaf damage is 4 cc / l.
Keywords---Pathogenicity, Bacillus thuringiensis, Tidal ecosystem, Plutella xylostella
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