Microscopic Changes of Ovaries in Relation to Inflammatory Mediators of Blood Plasma in Superovulated Rats

Authors

  • G. Banulata Universiti Putra Malaysia
  • S. Ganabadi
  • T. I. Azmi
  • H. Yaakub

Keywords:

Superovulation, Interleukin 8, Nerve Growth Factor, Prostaglandin E2, Inflammation

Abstract

This study was aimed to evaluate the microscopic changes that occur within the ovary and to assess the level of inflammatory mediators in blood plasma in rats that are superovulated. Eighteen female 12 weeks old Sprague Dawley rats were used in this study whereby histological sections of ovaries were examined to study the morphology of the ovary and blood analysis was carried out to analyse the inflammatory mediators in the blood plasma. The number of large follicles and healthy follicles were significantly increased (P<0.05) in superovulated rats but the diameter of the follicles were indifferent (P>0.05) when compared to control rats. The levels of Interleukin 8 (IL-8) was up regulated (P<0.05) at 8 hours after human Chorionic Gonadotropin (hCG) treatment but the Prostaglandin E2 (PGE2) and Nerve Growth Factor (NGF) showed insignificant differences (P>0.05) from control rats. It can be concluded from this study that IL-8 indicates increased level of inflammation in superovulated rats. The finding of this study in the increased level of IL-8 in superovulated rats is useful in further studies addressing problems in the superovulation treatment.

 

Author Biography

G. Banulata, Universiti Putra Malaysia

Department of Pre-clinical Sceince,

References

van Cappellen, W. A., Kramen, P., Laeuwen, E. C. M., Leeuw, R. and Jong, F. H. Induction of superovulation in cyclic rats by administration of decreasing doses of recombinant follicles stimulating hormone (Org32489), Human Reproduction, vol. 12, pp. 224-230, 1997.

Kon, H., Tohei, A., Hokkao, R. and Shinoda, M. Estrous Cycle Stage Independent Treatment of PMSG and hCG can Induce Superovulation in Adult Wistar Imamichi Rats, Experimant Animals, vol. 52, no. 2, pp. 185-187, 2005.

Lehloenya, K. C. and Greyling, J. P. C. The ovarian response and embryo recovery rate in Boer goat does following different superovulation protocols, during the breeding season, Small Ruminant Research, vol. 88, pp. 38-43, 2010.

Van der Auwera, I. and D'Hooghe, T. Superovulation of female mice delays embryonic and fetal development, Human Reproduction, vol. 16, pp. 1237–1243, 2001.

Forcadaa, F., Amer-Mezianeb, J. A., Abeciaa, M. C., Maurelc, J. A., Cebrián-Péreza, T., Muiño-Blancoa, B., Asenjod, M. I., Vázqueza, A. and Casaoa. Repeated superovulation using a simplified FSH/eCG treatment for in vivo embryo production in sheep, Theriogenology, vol. 75, pp. 769-776, 2011.

Richards, J. S., Liu, Z. and Shimada, M. Immune-like mechanism in ovulation, Trends in Endocrinology and Metabolism, vol. 19, pp. 191-196, 2008.

Chung, K. W., Ando, M. and Adashi, E. Y. Periovulatory and interleukin (IL)-1-dependent regulation of IL-6 in the immature rat ovary: A specific IL-1 receptor mediated eicosanoid- dependent effect, Journal of the Society for Gynecologic Investigation, vol. 7, pp. 301-308, 2000.

Chen, Y. J., Feng, Q. and Liu, Y. X. Expression of the steroidogenic acute regulatory protein and luteinizing hormone receptor and their regulation by tumor necrosis factor alpha in rat corpora lutea, Biology Reproduction, vol. 60, pp. 419-427, 1999.

Espey, L. L., Bellinger, A. S. and Healy, J. A. Ovulation: An Inflammatory Cascade of Gene Expression. In: Leung, P.C.K., Adasi, E.Y. eds. The Ovary. 2nd Ed. San Diego: Elsevier Academic Press. 145-165, 2004.

Zeineh, K., Kawano, Y., Fukuda, J., Nasu, H., Narahara, H. and Miyakawa, I. Possible modulators of IL-8 and GRO-alpha production by granulose cells, American Journal of Reproductive Immunology, vol. 50, pp. 98–103, 2003.

Frossard, N., Freund, V. and Advenier, C. Nerve Growth Factor and its receptors in asthma and inflammation, European Journal of Pharmacology, vol. 500, pp. 453-465, 2004.

Corbin, T. J. and McCabe, J. G. Strain Variation of Immature Female Rats in Response to Various Superovulatory Hormone Preparations and Routes of Administration, Contemporary Topics, vol. 41, no. 2, pp. 18-23, 2002.

Devine, P. J., Rajapaksa, K. S. and Hoyer, P. B. In vitro ovarian tissue and organ culture: A review, Frontiers in Biosciences, vol 1, pp. 1979–1989, 2002.

Junquiera, L. C., Carneiro, J. and Kelly, R. O. Basic Histology: Text and Atlas. Norwalk: Appleton and Lange (11th Edition), pp. 435-455, 1989.

Hay, M. F., Cran, D. G. and Moor, R. M. Structural changes occurring during atresia in sheep ovarian follicles, Cell and Tissue Research, vol. 169, pp. 515–529, 1976.

Debuljuk, L. Tachykinins in the normal and gonadotrophin- stimulated ovary of the mouse, Peptides, vol. 24, pp. 1445-1448, 2003.

Solomon, T., Largesse, Z., Mekbeb, A., Eyasu, M. and Asfaw, D. Effect of Rumex steudelii methanolic root extract on ovarian folliculogenesis and uterine histology in female albino rats, African Health Science, vol. 10, pp. 353-361, 2010.

Hsueh, A. J. W., McGee, E. A., Hayashi, M. and Hsu, S. Y. Hormonal regulation of early follicle development in the rat ovary, Molecular and Cellular Endocrinology, vol. 163, pp. 95–100, 2000.

Kagabu, S. and Umezu, M. Variation with age in the number of ovulated ova and follicles of Wistar-Imamiichi Adult rats Superovulated with eCG-hCG, Experimental Animal, vol. 55, no. 1, pp. 45-48, 2006.

Martín-Coello, J., González, R., Crespo, C., Gomendio, M. and Roldan, E. R. S. Superovulation and in vitro oocyte maturation in three species of mice (Mus musculus, Mus spretus and Mus spicilegus), Theriogenology, vol. 70, pp. 1004-1013, 2008.

Greenwald, S. G. How does Daily Treatment with Human Chorionic Gonadotropin Induce Superovulation in the cyclic hamster, Biology reproduction, vol. 48, pp. 133-142, 1993.

Panchal, S. and Nagori, C. B. Follicular monitoring, review article, Donald school journal of ultrasound in obstetrics, vol. 6, pp. 300-312, 2012.

Mannaerts, B., Uilenbroek, J., Schot, P. and De Leeuw, R. Folliculogenesis in Hyphosectomized Rats after treatment with Recombinant Human Follicle-Stimulating Hormone, Biology Reproduction, vol. 51, pp. 72-81, 1994.

Ono, M., Nakamura, Y., Tamura, H., Sugino, N. and Kato, H. Role of interleukin -1 beta in superovulation in rats, Endocrine Journal, vol. 44, pp. 797-804, 1997.

¬Levitas, E., Chamoun, D., Udoff, L. C., Andoo, M., Resnick, C. E. and Adashi, E. Y. Periovulatory and interleukin-1β-dependent up-regulation of intraovarian vascular endothelial growth factor (VEGF) in the rat: Potential role for VEGF in the promotion of periovulatory angiogenesis and vascular permeability, Journal of the Society for Gynecologic Investigation, vol. 7, pp. 51-60, 2000.

Sommer, C. and Kress, M. Recent findings on proinflammatory cytokines cause pain: peripheral mechanisms in inflammatory and neuropathic hyperalgesia, Neuroscience Letters, vol. 361, pp. 184-187, 2004.

Goto, J., Suganuma, N., Takata, K., Kitamura, K., Toshihiko, A., Kobayashi, H., Muranaka, Y., Furuhashi, M. and Kanayama, N. Morphological Analyses Of Interleukin-8 Effects On Rat Ovarian Follicles At Ovulation And Luteinization In Vivo, Cytokine, vol. 20, no. 4, pp. 168-173, 2002.

Fuji, A., Harada, T., Yamauchi, N., Iwabe, T., Nishi, Y. and Yanase, T. Interleukin-8 gene and protein expression are up-regulated by interleukin-1beta in normal human ovarian cells and a granulose tumor cell line, Fertility and Sterility, vol. 79, pp. 151–157, 2003.

Arici, A., Oral, E., Bukulmez, O., Buradagunta, S., Engin, O. and Olive, D. L. Interleukin-8 expression and modulation in human preovulatory follicles and ovarian cells, Endocrinology, vol. 137, pp. 3762–3769, 1996.

Ushigoe, K., Irahara, M., Fukumochi, M., Kamada, M. and Aono, T. Production and regulation of cytokine-induced neutrophil chemoattractant in rat ovulation, Biology of Reproduction, vol. 63, pp. 121-126, 2000.

Brännström, M. and Enskog, A. Leukocyte networks and ovulation, Journal of Reproductive Immunology, vol. 57, pp. 47-60, 2002.

Ujiko, T., Matsukawa, A., Tanaka, N., Matsuura, K., Yoshinaga, M. and Okamura, H. Interleukin-8 as an Essential Factor in the Human Chorionic Gonadotropin- Induced Rabbit Ovulatory Process: Interleukin-8 Induces Neutrophil Accumulation and Activation in Ovulation, Biology Reproduction, vol. 58, pp. 526-530. 1998.

Sugimoto, Y., Yamasaki, A., Segi, E., Tsuboi, K., Aze, Y., Nishimura, T., Oida, H., Yoshida, N., Tanaka, T. and Katsuyama, M. Failure of parturition in mice lacking the prostaglandin F receptor, Science, vol. 277, pp. 681–683, 1997.

Duffy, D. M. and VandeVoort, C. A. Maturation and Fertilization of Non-Human Primate Oocytes are Compromised by Oral Administration of a COX2 Inhibitor, Fertility and Sterility, vol. 95, pp. 1256-1260, 2011.

Faletti, A., Viggiano, J. M. and Gimeno, M. A. F. Beta-Endorphin Inhibits Prostaglandin Synthesis in Rat Ovaries and Blocks Induced Ovulation, Prostaglandins, vol. 49, pp. 93-103, 1995.

Espey, L. L. and Richards, J. S. Knobil and Neil’s Physiology of Reproduction; 3rd Edition. Chapter 11-Ovulation. pp. 425-74, 2006.

Faletti, A. G., Mohn, C., Farina, M., Lomniczi, A. and Rettori, V. Interaction among beta-endorphin, nitric oxide and prostaglandins during ovulation in rats, Reproduction, vol. 125, pp. 469-477, 2003.

Miller, S. B. Prostaglandins in Health and Disease, Seminars in Arthritis and Rheumatism, vol. 36, no. 1, pp. 37-49, 2006.

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Published

2014-08-14

How to Cite

Banulata, G., Ganabadi, S., Azmi, T. I., & Yaakub, H. (2014). Microscopic Changes of Ovaries in Relation to Inflammatory Mediators of Blood Plasma in Superovulated Rats. Asian Journal of Applied Sciences, 2(4). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/1505

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