Efficacy of Different Anaesthetics on Depth of Anaesthesia and Hematological Parameters of Pigs
DOI:
https://doi.org/10.24203/ajafs.v10i6.7101Keywords:
Anesthesia, Body temperature, Haematological indices, PigsAbstract
Four groups of piglets were included in the experiment. In each of the experiments, we applied azaperone as the first premedication drug in the 2nd group 4 mg/kg body weight and in the other groups 5 mg/kg body weight. In experiment no. 1, we subsequently administered diazepam (1 mg/kg, i.m.) and ketamine (10 mg/kg, i.m.) to the piglets. In experiment no. 2, we applied xylazine (2 mg/kg, i.m.) and ketamine (20 mg/kg, i.m.). In experiment no. 3 we used diazepam (1 mg/kg, i.m.), xylazine (2 mg/kg, i.m.) and butorphanol (0.2 mg/kg, i.m.). In experiment no. 4, we administered xylazine (2 mg/kg, i.m.) and butorphanol (0.2 mg/kg, i.m.) to piglets. We applied the mentioned drug combinations intramuscularly in the area of the neck muscles. Subsequently, in each of the experiments, we performed epidural anesthesia using 2% lidocaine, in a dose of 1 ml/40 cm of body length.
After putting the animals under anesthesia, we recorded breathing and pulse frequencies, body temperature and checked reflex responses at time intervals. We performed a zero blood collection before the application of the first drug, the first collection after anesthesia and then a second collection the next day, in order to determine the hematological profile. From the conducted experiments, we assessed the relationship of the used combinations of substances to the course and depth of anesthesia and, according to the hematological profile, we evaluated the effect of these substances on specific hematological parameters.
We recorded the longest duration of anesthesia in the first experiment when one of the piglets had disappeared pedal reflexes even after 90 minutes. On the contrary, the shortest anesthesia was during the third experiment, when all piglets reacted 60 minutes post-lidocaine. During experiments 1 and 3, we noticed a significant drop in body temperature compared to the reference values. In a short time interval after the lidocaine administration, the reflex of the pelvic limbs completely disappeared in all tested subjects in each of the experiments. In experiment no. 3, at time 5 minutes post lidocaine administration, the nasal reflex and the reflex of the forelegs were also negative in all animals. In all tested subjects, 30 minutes after the epidural anesthesia was performed, we observed a negative reflex of the pelvic legs during the second and third trials. During experiment no. 3, we also observed a negative nasal reflex and a reflex of the front legs in the majority of the tested animals in the mentioned time interval.
References
Adetunji A. , Osunbunmi, O. T. Haematological effest od azaperone sedation in pigs. Africal Journal of Biomedical Research. ISSN 1119 – 5096, vol. 3, no. 2, pp. 131 – 133, 2000.
Blaze C. Anesthesia and Care of the Large Animal for Survival Studies: Pig. 9. 1. 2019: < https://isctr.org/chapter-i-6/>
Boschert K., Flecknell P. A., Fosse R. T., Framstad T., Ganter M., Sjøstrand U., Stevens J., Thurman J. Ketamine and its use in the pig. Laboratory Animals. ISSN 1758 – 1117, vol. 30, no. 3, pp. 209 –219, 1996.
Calzetta L., Rossi P., Bove P., Alfonsi P., Bonizzi L., Roncada P., Bernardini R., Ricciardi E., Montuori M., Pistocchini E., Mauti P., Mattei M. A Novel and Effective Balanced Intravenous-Inhalant Anaesthetic Protocol in Swine by Using Unrestricted Drugs. Experimental Animals. ISSN 1881 – 7122, vol. 63, no. 4, p. 423 – 433, 2014.
Čonková, E. Veterinary pharmacology (in Slovak), 1st edition, Košice, Slovakia: University of Veterinary Medicine and Pharmacy, ISBN 978-80-8077-103-4, 280 p., 2008
Hannon J. P., Bossone C. A., Wade C.E. Normal physiological values for conscious pigs used in biomedical research. Laboratory Animal Science. ISSN 0023-6764, vol. 40, no. 3, p. 293 – 298, 1990.
Harikrishnan V. S., Shenoy S. J., Umashankar P. R. Anaesthetic regimen for coronary stenting in porcine model. The Indian veterinary journal. ISSN 0019 – 6479, vol. 83, no. 5, p. 486 – 489, 2006.
Janyce L, Cornick – Seahorn. Veterinary anesthesia. United States of America: Elsevier - Health Sciences, ISBN 0-7506-7227-7, 304 p., 2000.
Kaiser G. M., Heuer M. M., Frühauf N. R., Kühne Ch. A., Broelsch Ch. E.. General Handling and Anesthesia for Experimental Surgery in Pigs. Journal of Surgical Research. ISSN 0022 – 4804, vol. 130, no. 1, p. 73- 79, 2006.
Kayode, O. A. Studies on Epidural anaesthesia using lidocaine with Adrenaline on Hemato-biochemical responses in pregnant West African Dwarf goats. In Journal of Medical Research. ISSN 2276 – 9900, vol. 6, no. 2, pp. 12 – 15, 2017.
Kohn D. F.. Anaesthesia and analgesia in laboratory animals: American College of Laboratory Animal Medicine Series. 1st edition, Unites states of America : Academic Press, ISBN 9780080527222, 1997.
Kováč, G. Diseases of pigs (in Slovak). Prešov, Slovakia: Vydavateľstvo Michala Vaška, ISBN 978-80-7165-839-9, 980p, 2011.
Lugo – Roman L. A., Rico P. J., Sturdivant R., Burks R., Settle T.L. Effects of serial anesthesia using ketamine or ketamine/medetomidine on hematology and serum biochemistry values in rhesus macaques. Journal of Medical Primatology. ISSN 1600 – 0684, vol. 39, no. 1, p. 41 – 49, 2010.
Sakaguchi M., Nishimura R., Sasaki N., Ishiguro T., Tamura H., Takeuchi A. Sedative Effects of Medetomidine in Pigs. Journal of Veterinary Medical Science. ISSN 1347 – 7439, vol. 54, no. 4, p. 643 – 647, 1992.
Suresha L., Ranganath B. N., Vasanth M. S., Ranganath L. Haemato-biochemical studies on triflupromazine HCL and diazepam premedication for propofol anaesthesia in dogs. Veterinary world. ISSN 0972 – 8988, vol. 5, no. 11, p. 672 – 675, 2012.
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