Morphological and Molecular Characterization of Natural Entomopathogenic Fungi
Keywords:
entomopathogenic fungi, soil, molecular identification, Beauveria bassianaAbstract
In this study, entomopathogenic fungi were isolated from soil samples collected from the central villages of Kahramanmaras wheat fields and Baskonus forests. Development characteristics of isolates on artificial medium, microscopic examination of the morphological structures and molecular characterization were performed. Observed sequence results were compared with database sequence using Blast and phylogenetic tree was drawn. Beauveria bassiana (14), Fusarium oxysporum (8), F. solani (1), Aspergillus flavus (4) and Penicillium sp. (1) were identified as entomopathogenic fungi species in given area.References
Lacey L A, Frutos R, Kaya H K, Vail P, “Insect pathogens as biological control agents: Do they have a future?†Biological Control, vol. 21, pp. 230-248, 2001.
Shahid M R, Farooq J, Mahmood A, Ilahi F, Riaz M, “Seasonal occurrence of sucking insect pest in cotton ecosystem of Punjab, Pakistan.†Journal of Advanced Scientific Research, vol.4, pp. 26-30, 2012.
Keller S, Zimmerman G, Mycopathogens of soil insects. In: Wilding, N., Collins, N.M., Hammond, P.M., Webber, J.F. (Eds.), Insect-Fungus Interactions. Academic Press, London, pp. 240–27, 1989.
Jackson T A, Alves S B, Pereira R M, Success in biological control of soil-dwelling insects by pathogens and nematodesâ€. In: Biological control: Measures of success (Ed: Gurr G, Wratten). Academic Press, London, pp 271–29, 2000.
Meyling N V, Eilenberg J, “Occurrence and distribution of soil borne entomopathogenic fungi within a single organic agroecosystem.†Agriculture, Ecosystems & Environment, vol.113, pp. 336–341, 2006.
Quesada-Moraga E, Navas-Corte´s J A, Maranhao E A A, Ortiz-Urquiza A, Santiago-A´ lvarez C, “Factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soilsâ€. Mycological Research, vol.111, pp. 947–966, 2007.
Bidochka M J, Kasperski J E, Wild G A M, “Occurrence of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana in soils from temperate and near-northern habitatsâ€. Canadian Journal of Botany, vol.76, pp. 1198-1204, 1998.
Ali-Shtayeh M S, Mara A B B M, Jamous R M, “Distribution, occurrence and characterization of entomopathogenic fungi in agricultural soil in the Palestinian areaâ€. Mycopathologia, vol. 156, pp. 235-244, 2002.
Keller S, Kessler P, Schweizer C, “Distribution of insect pathogenic soil fungi in Switzerland with special reference to Beauve, ria brongniartii and Metarhizium anisopliaeâ€, BioControl, vol.48, pp. 307-319, 2003.
Vänninen I, Tyni-Juslin J, Hokkanen H, “Persistence of augmented Metarhizium anisopliae and Beauveria bassiana in Finnish agricultural soilsâ€, BioControl, vol.4. pp. 201-222, 2000.
Sevim A, Demir I, Höfte M, Humber R A, DemirbaÄŸ Z, “Isolation and characterization of entomopathogenic fungi from hazelnut-growing region of Turkeyâ€, BioControl, vol.55, no.2, pp. 279-297, 2010.
Er K M, “Recovering entomopathogenic fungi in Pistachio orchards in Gaziantep, Adıyaman and KahramanmaraÅŸâ€. Turkish Journal of Biological Control, vol.4, no. 2, pp. 155-163, 2013.
Koz C, Güven Ö, “Entomopathogenic fungi isolated from wheat fields in villages of KahramanmaraÅŸ city centerâ€. Turkish Journal of Biological Control, vol.5, no. 1, pp. 39-51, 2014.
Izgi Ãœ, Güven Ö, “Entomopathogenic fungi isolated from soil samples taken from BaÅŸkonuÅŸ forestland in KahramanmaraÅŸâ€. Plant Protect Bullutein, vol. 54, no.3, pp. 201-209, 2014.
Samson R A, “Identification – Entomopathogenic Deuteromycetes. In: Microbial Control of Pests and Plants Diseases (Ed: Burges H. D.), 1970–1980. Acad. Press, London, pp.93–106, 1981.
Castrillo L A, Brooks W M, “Differentiation of Beauveria bassiana isolates from the darkling beetle, Alphitobius diaperinus, using isozyme and RAPD analysesâ€. Journal of Invertebrate Pathology, vol.72, pp. 190-196, 1998.
Wang C S, Li Z Z, Butt T M, “Molecular studies of co-formulated strains of the entomopathogenic fungus, Beauveria bassianaâ€. Journal of Invertebrate Pathology. Vol.80, pp. 29-34, 2002.
Aquino De Muro A, Elliott S, Moore D, Parker B L, Skinner M, “Molecular characterization of Beauveria bassiana isolates obtained from overwintering sites of Sunn Pests (Eurygaster and Aelia species)â€. Mycological Research, vol. 109, no. 3, pp. 294–306, 2005.
Estrada M E, Camacho M V, Benito C, “The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (Issrs)â€. Cellular & Molecular Biology Letters. Vol.12, pp. 240 – 252, 2007.
Zimmermann G, “The Galleria bait method for detection of entomopathogenic fungi in soilâ€. Applied Entomology and Zoology, vol.102, pp. 213-215, 1986.
Goettel M S, Inglis G D, Fungi: Hyphomycetes. In: Manual of Techniques in Insect Pathology Lacey, L.A. (Ed.), Academic Press, San Diego, USA, pp. 213–249, 1997.
Humber R, Fungi: identiï¬cation. In: Manual of Techniques in Insect Pathology. Lacey, L.A. (Ed.), Academic Press, San Diego, pp 153–185, 1997.
Sambrook J, Fritsch E F, Maniatis T, Molecular cloning: a laboratory manual, vol. 3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 1989.
Minitab, Release 15., Minitab Inc. 3081 Enterprise Drive State College, PA, USA. 1993.
Er K M, Mart C, “Some entomopathogenic fungi recovered in KahramanmaraÅŸ province and evaluation of the province for using entomopathogenic fungiâ€. Kahramanmaras Sutcu Imam University Journal of Natural Sciences, vol.12, no.2, pp. 52-58, 2009.
Sun B D, Liu X Z, “Occurrence and diversity of insect-associated fungi in natural soils in Chinaâ€. Applied Soil Ecology. Vol.39, pp.100–108, 2008.
Sun B D, Yu H Y, Chen A J, Liu X Z, “Insect-associated fungi in soils of field crops and orchardsâ€. Crop Protection, vol.27, pp. 1421–1426, 2008.
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