Allele and Genotype Frequencies of the ABO Blood Group System in a Palestinian Population


  • Lamia'a S. Saqer Medical Sciences Department-College of Science and Technology, Khan Younis
  • Fadel A. Sharif Islamic University of Gaza


ABO alleles, ABO genotypes, AS-PCR, Palestine


The ABO blood group system is one of the most important blood type in clinical practice. Currently, there is no information on the ABO genotype and allele frequency from Gaza strip. Data from this study will be useful for workers and the field and for future clinical applications of ABO genotyping in our area.

This study was conducted in order to determine the major ABO alleles' and genotypes' frequencies in a Palestinian population residing in Gaza Strip.

A four separate–reaction multiplex allele specific polymerase chain reaction (AS-PCR) was used to determine the ABO genotypes of 201 unrelated subjects whose DNA, extracted from peripheral blood, was subjected to genotyping.

The study revealed the following genotypes:  A1A1 (n=3), A1O 1 (n=24), A1O2 (n=25), A1A2 (n=4), A2A2 (n=2), A2O1 (n=13), A2O2 (n=2), B1B1 (n=5), B1O1 (n=26), B1O2 (n=14), A1B (n=11), A2B (n=4) , O1O1 (n=31) , O1O2 (n=26) and O2O2 (n=11), from which the deduced phenotypes were: A (n=73), B (n=45), AB (n=15) and O (n=68). The frequencies of the A1 , A2 , B1, O1, O2alleles were: 0. 174, 0.067, 0.162, 0.376 and 0.221, respectively. 

There was no deviation between genotype-derived phenotypes results and serologically determined phenotypes. Additionally, the genotype distribution was in Hardy-Weinberg equilibrium.

ABO genotyping must be considered when determining ABO phenotypes, in blood transfusion, tissue/organ transplantation, blood typing discrepancies and forensic/paternity testing investigations.

Author Biography

Fadel A. Sharif, Islamic University of Gaza

Department of Medical Laboratory Sciences

Professor of Molecular Biology


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How to Cite

Saqer, L. S., & Sharif, F. A. (2013). Allele and Genotype Frequencies of the ABO Blood Group System in a Palestinian Population. Asian Journal of Pharmacy, Nursing and Medical Sciences, 1(4). Retrieved from