ABO Genotype and Risk of Recurrent Pregnancy Loss in Carriers of FVL in Palestinian Population


  • Lamia'a Sobhi Saqer University Collage Of Science And Technology


Recurrent pregnancy loss, factor V Leiden, ABO genotypes


The aim of this study was to define ABO genotypes in the Palestinian population and to assess the impact of ABO blood group genotypes and FV Leiden and we investigated whether predisposition to RPL was higher in non-OO genotype carriers than in OO genotype carriers. The study included 209 subjects suffered from RPL : 85 FV Leiden carriers as case group and 124 as a control group. Genotyping to 5 common alleles of ABO blood groups was performed by allele specific polymerase chain reaction with specific primers (AS-PCR). The results showed that there was no association between non-OO blood group genotypes and the risk of RPL(odds ratio [OR] 1.4, 95% confidence interval [95% CI] 0.74-2.6). Comparison of OO genotype and non-OO genotype carriers between case and control subjects yielded a higher OR in A1A1 / A1A2 blood group genotypes (OR: 2.2, 95% CI: 0.6- 8.19), followed by A1O1 / A1O2 (OR:1.6,95% CI: 0.77-3.55) B1B1/ B1O1/ B1O2 (OR: 1.3, 95% CI: 0.6-2.76) and A1B/ A2B (OR: 1.2, 95% CI: 0.33-4.00) which did not reach statistical significance. The results of ABO genotyping showed no statistically significant difference in the frequency of OO and non-OO genotype carriers between the case group who had FVL and the control group (OR: 1.4, 95% CI : 0.74-2.6).



Author Biography

Lamia'a Sobhi Saqer, University Collage Of Science And Technology

Lecuturer at The Medical Department


Yamamoto F. Molecular genetics of the ABO histo-blood group system. Vox Sang. 1995;69:1.

Yamamoto F, Clausen H, White T, Marken J, Hakamori S. Molecular genetic basis of the histoblood group system. Nature. 1990;345:229-233.

Yamamoto F, McNeill PD, Hakamori S. Genomic organization of human histo-blood group ABO genes. Glycobiology. 1995;5:51-58.

Bennett EP, Steffensen R, Clausen H, Weghuis DO, van Kessel AG. Genomic cloning of the human histo-blood group ABO locus. Biochem Biophys Res Comm. 1995;206:318-325.

Malekasgar A. ABO blood group prevalence in spontaneously repeated abortion .Turk Journal Haematol 2004;21: 181-187 .

Saito S. The causes and treatment of recurrent pregnancy loss . Journal of the Japan Medical Association 2009;52: 97–102.

Mierla D., Szmal C., Neagos D., Cretu R., Stoian V., Jardan D. Association of Prothrombin (A20210G) and Factor V Leiden (A506G) with Recurrent Pregnancy Loss. Maedica, 2012; 7 :222-226 .

Brenner B, Sarig G, Weiner Z, Younis J,Blumenfeld Z, Lanir N . Thrombophilic polymorphisms are common in women with fetal loss without apparent cause. Journal Thromb Haemost 1999; 82:6-9.

Carrington B, Sacks G, Regan L . Recurrent miscarriage: pathophysiology and outcome. Curr Opin Obstet Gynecol 2005; 17: 591-597.

Doyle NM, Monga M . Thromboembolic disease in pregnancy. Obstet Gynecol Clin North Am 2004; 31: 319-344.

Jenkins PV, O’Donnell JS. ABO blood group determines plasma von Willebrand factor levels: a biologic function after all? Transfusion.2006; 46(10):1836-1844.

Tregouet DA, Heath S, Saut N, Andreani C, Schved JF, Pernod G, Galan P, Drouet L Zelenika D, Vague I ,Alessi MC ,Tiret L ,Lathrop M, Emmerich J, Morange PE Common susceptibility alleles are unlikely to contribute as strongly as the FV and ABO loci to VTE risk: results from a GWAS approach. Blood.2009; 113(21):5298-5303.

Jick H, Slone D, Westerholm B, Inman WH, Vessey MP, Shapiro S, et al. Venous thromboembolic disease and ABO blood type. A cooperative study. Lancet. 1969;1:539-542.

Crawley JT, Lane DA,WoodwardM, RumleyA, LoweGD. Evidence that high von Willebrand factor and low ADAMTS-13 levels independently increase the risk of a non-fatal heart attack. Journal Thromb Haemost 2008; 6: 583–588.

Folsom AR, RosamondWD, Shahar E, Cooper LS, Aleksic N, Nieto FJ, Rasmussen ML, Wu KK. Prospective study of markers of hemostatic function with risk of ischemic stroke. The Atherosclerosis Risk in Communities (ARIC) Study Investigators. Circulation 1999; 100: 736–742.

Kraaijenhagen RA, in_t Anker PS, Koopman MM, Reitsma PH, Prins MH, van den Ende A, Buller HR. High plasma concentration of factor VIIIc is a major risk factor for venous thromboembolism. Journal Thromb Haemost 2000; 83: 5–9.

Ohira T, Cushman M, Tsai MY, Zhang Y, Heckbert SR, Zakai NA, Rosamond WD, Folsom AR. ABO blood group, other risk factors and incidence of venous thromboembolism: the Longitudinal Investigation of Thromboembolism Etiology (LITE). Journal Thromb Haemost 2007; 5: 1455–1461.

Tanis B, Algra A, van der Graaf Y, Helmerhorst F, Rosendaal F. Procoagulant factors and the risk of myocardial infarction in young women. Eur Journal Haematol 2006; 77: 67–73.

Tracy RP, Arnold AM, Ettinger W, Fried L,Meilahn E, Savage P. The relationship of fibrinogen and factors VII and VIII to incident cardiovascular disease and death in the elderly: results from the cardiovascular health study. Arterioscler Thromb Vasc Biol 1999; 19: 1776–1783.

Preston AE, Barr A. The plasma concentration of factor VIII in the normal population. Br Journal Haematol 1964;10:238–245.

Lamia'a S. Saqer and Fadel A. Sharif Allele and Genotype Frequencies of the ABO Blood Group System in a Palestinian Population .Asian Journal of Pharmacy, Nursing and Medical Sciences 2013; 01: 98 – 103 .

Wautrecht, J.C., Galle, C., Motte, S., Dereume, J.P. & Dramaix, M. The role of ABO blood group in the incidence of deep vein thrombosis. Thrombosis and Haemostasis.1998; 79, 688–689.

Parand, A., Zolghardi, J., Nezam, M., Afrasiabi, A., Haghpanah, S. and Karimi, M. Inherited Thrombophilia and Recurrent Pregnancy Loss. Iranian Red Crescent Medical Journal 2013;15.




How to Cite

Saqer, L. S. (2016). ABO Genotype and Risk of Recurrent Pregnancy Loss in Carriers of FVL in Palestinian Population. Asian Journal of Pharmacy, Nursing and Medical Sciences, 4(1). Retrieved from https://ajouronline.com/index.php/AJPNMS/article/view/3547