Alignment of E. coli Bacteria in a PDMS Microfluidic Channel using Ultrasonic Standing Wave
Keywords:
ultrasonic standing wave, live cell manipulation, PDMS microfluidic channel, e.coli bacteriaAbstract
Ultrasonic radiation force caused by ultrasonic standing wave has the capability to align microparticles suspended in fluid medium in its nodal or antinodal planes depending on the mechanical properties of particles and fluid medium. In some of the recent works, alignment of live cells like Cyanobacteria etc. inside a biocompatible Polydimethylsiloxane (PDMS) microfluidic channel were successfully carried out by utilizing the ultrasonic radiation force. This study is an extended work of these types of ultrasonic live cell manipulation in PDMS microfluidic channel. In this work, we demonstrate the alignment of E.coli bacteria, which is widely used in biotechnological research area, using ultrasonic radiation force in a PDMS microfluidic channel. With this successful alignment of E.coli bacteria it is proved that ultrasonic standing wave has the vast range of manipulation capability of different types of live cells.
References
A. Ashkin, “Acceleration and trapping of particles by radiation pressure,†vol. 24, no. 4, pp. 156-159, 1970.
A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, and S. Chu, “Observation of a single-beam gradient force optical trap for dielectric particlesâ€, Optics Letters, vol. 11, no. 5, pp. 288-290, 1986.
J. E. Molloy, M. J. Padgett, “Lights, action: Optical tweezersâ€, Contemporary Physics, vol. 43, no. 4, pp. 241-258, 2002.
K. Dholakia, P. Reece, M. Gu, “Optical micromanipulationâ€, Chem. Soc. Rev., vol. 37, no.1, pp. 42-55, 2008.
J. Jung, K. -H. Han, “Lateral-driven continuous magnetophoretic separation of blood cellsâ€Appl. Phys. Lett. 93, 223902, 2008.
K. -H. Han, A. B. Frazier, “Paramagnetic capture mode magnetophoretic microseparator for high efficiency blood cell separationsâ€, Lab Chip, vol. 6, pp. 265–273, 2006.
M. D. Vahey and J. Voldman, “An Equilibrium Method for Continuous-Flow Cell Sorting Using Dielectrophoresisâ€, Anal. Chem., vol. 80, no. 9, pp. 3135–3143, 2008.
N. Gadish, J. Voldman, “High throughput positive dielectrophoretic bioparticle microconcentratorâ€, Anal. Chem, vol. 78, no. 22, pp.7870-7876, 2006.
T. Kozuka, T. Tuziuti, H. Mitome, T. Fukuda, “One dimensional transportation of particles using an ultrasonic standing waveâ€, 6th int. symp. Micro Machine and Human Science Proc., pp.179-185, 1995.
D. A. Johnson, and D. L. Feke, “Methodology for fractionating suspended particles using ultrasonic standing wave and divided flow fieldsâ€, Separations Technology, vol. 5, no. 4, pp. 251-258, 1995.
J. J. Hawkes, W. T. Coakley, “A continuous flow ultrasonic cell-filtering methodâ€, Enzyme and Microbial Technology, vol. 19, no. 1, pp. 57-62, 1996.
J. J. Hawkes, W. T. Coakley, “Force field particle filter, combining ultrasound standing waves and laminar flowâ€, Sensors and Actuators B: Chemical, vol. 75, no. 3, pp. 213-222, 2001.
A. Nilsson, F. Petersson, H. Jönsson, T. Laurell, “Acoustic control of suspended particles in microfluidic chipsâ€, Lab Chip, vol. 4, no. 2, pp. 131-135, 2004.
F. Petersson, L. Aberg, A. M. Sward-Nilsson, T. Laurell, “Free flow acoustophoresis:  Microfluidic-based mode of particle and cell separationâ€, Anal. Chem, vol. 79, no. 14, pp. 5117-5123, 2007.
M. Evander, A. Lenshof, T. Laurell, J. Nilsson, “Acoustophoresis in wet-etched glass chipsâ€, Anal. Chem, vol. 80, no. 13, pp. 5178-5185, 2008.
P. Augustsson, B. A. Lena, K. Ann-Margret, S. Nilsson. T. Laurell, “Buffer medium exchange in continuous cell and particle streams using ultrasonic standing wave focusingâ€, Microchim Acta. vol. 164, no. 3-4, pp. 269-277, 2009.
F. Petersson, A. Nilsson, C. Holm, H. Jonsson, T. Laurell, “Separation of lipids from blood utilizing ultrasonic standing waves in microfluidic channelsâ€, Analyst. vol. 129, no. 10, pp. 938-943, 2004.
T. J. Johnson, D. Ross, M. Gaitan, L.E. Locascio, “Laser Modification of Preformed Polymer Microchannels:  Application To Reduce Band Broadening around Turns Subject to Electrokinetic Flowâ€, Anal. Chem. vol. 73, no. 15, pp. 3656–3661, 2001.
T. Franke, S. Braunmuller, L. Schmid, A. Wixforth and D. A. Weitz, “Surface acoustic wave actuated cell sorting (SAWACS)â€, Lab Chip, vol. 10, no. 6. pp. 789–794, 2010.
A. T. Islam, A. H. Siddique, T. S. Ramulu, V. Reddy, Y. J. Eu, S. H. Cho, C. J. Kim, “Ultrasonic alignment of bio-functionalized magnetic beads and live cells in PDMS microfluidic channelâ€, Biomedical Microdevices, vol. 14, no. 6, pp. 1077-1084, 2012.
K. Yosioka, Y. Kawasima, “Acoustic radiation pressure on a compressible sphereâ€, Acustica, vol. 5, no. 3, pp. 167-173, 1955.
Andrea Thompson, "E. coli Thrives in Beach Sands", Live Science. (2007-06-04)
S. Ishii, M. J. Sadowsky, "Escherichia coli in the Environment: Implications for Water Quality and Human Health". Microbes and Environments, vol. 23, no. 2 pp. 101-108 , 2008.
Downloads
Published
Issue
Section
License
- Papers must be submitted on the understanding that they have not been published elsewhere (except in the form of an abstract or as part of a published lecture, review, or thesis) and are not currently under consideration by another journal published by any other publisher.
- It is also the authors responsibility to ensure that the articles emanating from a particular source are submitted with the necessary approval.
- The authors warrant that the paper is original and that he/she is the author of the paper, except for material that is clearly identified as to its original source, with permission notices from the copyright owners where required.
- The authors ensure that all the references carefully and they are accurate in the text as well as in the list of references (and vice versa).
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
- The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.
