The Application of Endophytic <i>Trichoderma Viride</> PS-3.7 Using Indigenous Tidal Swamp Aquatic Weeds Media to Control Paddy Wilt Disease


  • Ismed Setya Budi, Mariana Faculty of Agriculture Lambung Mangkurat University


Trichoderma, aquatic weed, wilt disease, swamps land


Characteristic of paddy at tidal swamp should passed taradak, ampak and lacak phase. Disease control by using synthetic pesticides sometimes constrained by land inundated. This research tries to antagonists and aquatic weeds utilizing site-specific swamps land. The result of the study giving evidence  the best antagonist (Trichoderma viride PS-3.7) with aquatic weeds used as applicative media are considered to be able to increase the number of antagonistic spores, inhibiting, and antagonistic potential toward wilt disease in paddies caused by Fusarium oxysporum. Then, the number of spores produced in enceng gondok (Eichhornia crassipes),, kiambang (Salvinia molesta)  and kayapu (Pistia stratatiotes) were (7.80 x 108 spora/g), (6.1 x 108 spora/g), (2.98 x 108 spora/g), respectively. The inhibiting capability of Trichoderma viride isolate PS-3.7 toward F. oxysporum was 80.44%, while it reached 90.22% toward Fusarium oxysporum. The results of the observation revealed that in taradak phase where the applicative medium of water hyacinth was employed, the intensity of disease was 15.71%, ampak phase, where kiambang was introduced, the disease intensity was 20.00%, while in lacak phase with the application of water hyacinth, the disease intensity was only as much as 10.70% in comparison with the control group which reached 45.60%.


Budi, I.S. dan Mariana, 2010. Pengendalian hayati penyakit busuk batang padi di lahan pasang surut Kalimantan Selatan dengan memanfaatkan jamur antagonis rizosfir. Fakultas Pertanian Unlam Banjarbaru.

Carling, D.E. and D.R. Sumner. 1993. Rhizoctonia. Methods for Research on soilborne Phytopathogenic Fungi. The Amer. Phytopathol. Soc. Minnesota. p. 157-165.

Fokkema, N.J., J.H. Bond and H.A. Fribourg. 1959. Methods for Studying Soil Microflora Plant Disease Relationships. Burgess Publ. Co. USA. pp. 247.

Abadi, A., 1987. Biologi Ganoderma bonanensis pada kelapa sawit (Elaeis guenensis Jcq) dan pengaruh beberapa mikfoba antagonis terhadap pertumbuhannya. Disertasi. Pasca Sarjana Institut Pertanian Bogor.

Chao, W.L., E.B. Nelson, G.E. Harman and H.C. Hoch. 1986. Colonization of the Rhizosphere by Biological Control Agent Applied to Seed. Phytopathol. 96:60-65.

Bakker P.A.H.M, 1987. Bioassay for studying the roleof siderophores in potato growth stimulation by pseudomonas spp in short potato rotations. Soil Biol Biochem 19 (4): 443-449.

Suhaya, I., 1991. Pengaruh Berbagai Kandungan Eceng Gondok (Eichornia Crassipes) Terhadap Pertumbuhan Dan Koefisien Nilai Nutrisi Ikan Nila Merah (Oreochromis Sp. Universitas Pajajaran, Bandung. 47 hal.

Hjeljord, L. G., Stensvand, A., and Tronsmo, A. 2001. Antagonism of nutrient-activated conidia of Trichoderma harzianum (atroviride) P1 against Botrytis cinerea. Phytopathology 91:1172-1180.

Paustian, K. and Schnurer, J. 1987. Fungal growth response to carbon and nitrogen limitation: a theoretical model. Soil Biol Biochem, 19: 613-620.

Whipps, J.M. 2001. Microbial Interactions and biocontrol in the rhizosphere. Journal of experimental botany 52 : 487 – 511.

Simons, M., H. P. Permentier, L. A. de Weger, C. A. Wijffelman, and B. J. J. Lugtenberg. 1997. Amino acid synthesis is necessary for tomato root colonization by Pseudomonas fluorescens strain WCS365. Mol. Plant-Microbe Interact.10:102–106.

Horst, L., Locke, J.C., Krause, C.R., Mcmahon, R.W., Madden, L.V., Hoitink, H.A. 2005. Suppression of botrytis blight of begonia by trichoderma hamatum 382 in peat and compost-amended potting mixes. Plant Disease. 89:1195-1200.

Nawawi, I.A., 2013. Pengaruh Aplikasi Kompos Jerami, Konsorsium Pupuk Hayati Dan Trichoderma sp. terhadap Intensitas Penyakit pada Tanaman Padi Sistem Intensifikasi Padi Aerob Terkendali Berbasis Organik (IPAT-BO).

Kumar, A, 2003. Fungal biocontrol agents (BCAS) and their metabolies. Departement of Biological Science, Pant University of Agriculture and Technology, Pantnagar.

Pal K.K. and B. Mc S Gardener, 2006. Biological control of plant pathogen. The Plant Health Instructor. Biological Control.aspx.

Vey A, Hoagland RE dan Butt TM .2001. Toxic metabolites of fungal biocontrol agents. Fungi as Biocontrol Agents: Progress,Problems and Potential (Butt TM, Jackson C &Magan N, eds), pp. 311–346. CAB International, Bristol.

Xiao-Yan, S. S Qing-Tao, XieShu-Tao, Chen Xiu-Lan, Sun Cai-Yun and Zhang Yu-Zhong. 2006. Broad-spectrum antimicrobial activity and high stability of Trichokoninsfrom Trichodermakoningii SMF2 against plant pathogens. FEMS MicrobiolLett 260 (2006).

El-Katatny M. H.W. Smitch, K.H. Robra, M.S and GM Gubi. 2000: Production of Chitinase and-1,3-glucanase by T. harzianum for control of the phytopathogenic fungus Sclerotium rolfsii. Food technol. biotechnol. 38 (3) 173–180.

Mostapha N. K. 2004. Biological Control of Rhizoctonia solani, the Causal Agent of Rice Sheath Blight by Antagonistics Bacteria in Greenhouse and Field Conditions. Plant Pathology Journal, 3: 88-96.

Hartaman G.E., Hayes C.K., Lorito M., Broadway R.M., Dipietro A., Peterbauer C., Tronsmo 1992. Chitinolytic enzymes of Trichoderma harzianum: purification Of chitobiosidase and endochitinase. Phytopathology. 83:313–318.

Nelson E. B, 1988. Enhancemant of Trichoderma induced biological control of pythium seed rot and pre emergence damping off of peas. Soil Biol Biochem 20 (2): 145-150.

Mendes R. 2007. Diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates.

Ozbay N and Newman S. E. 2004. Biological control with Trichoderma spp. with emphasis on T. Journal of Biological Science 7 (4): 478-484.


How to Cite

Mariana, I. S. B. (1). The Application of Endophytic <i>Trichoderma Viride</> PS-3.7 Using Indigenous Tidal Swamp Aquatic Weeds Media to Control Paddy Wilt Disease. Asian Journal of Applied Sciences, 3(1). Retrieved from