Toll-Like Receptor-2 Gene [TLR2] Polymorphism and Susceptibility to Mycobacteria Tuberculosis Infections in Sudanese Patients
Keywords:
Background & Aim, Tuberculosis [TB] is a major public health threat and a leading cause of death worldwide. Innate immunity plays an important role in the host defense against M. tuberculosis through recognition Toll-Like Receptors [TLRs]. PolymorphismAbstract
Tuberculosis [TB] is a major public health threat and a leading cause of death worldwide. Innate immunity plays an important role in the host defense against M. tuberculosis through recognition Toll-Like Receptors [TLRs]. Polymorphisms in TLR2 [Arg753Gln] gene have been reported to be associated with increased susceptibility to TB. This study aimed to determine possible roles for TLR2 [Arg753Gln] gene polymorphism in susceptibility to M. tuberculosis infections in a cohort of Sudanese individuals. Following informed consent, 141 individuals with smear positive tuberculosis and latent TB infections [mean age 19±38.3 years; male: female ratio  2:1] and 197  apparently healthy individuals [mean age 30±14; male female ration of 1:5] were enrolled. DNA was extracted from EDTA-blood using the guanidine chloride method. PCR was carried out using TLR2 gene specific primers, Forward: [5'CATTCCCCAGCGCTTCTGCAAGCTCC-3'] and Reverse: [5'GGAACCTAGGACTTTATCGCAGCTC-3')]. Genotyping was done by RFLP using MSP1restriction enzyme. The TLR2 Arg753Gln mutant allele was not detected in any of the study groups. TLR2 Arg753Gln gene polymorphisms plays little or no role in host susceptibility to Mycobacterium tuberculosis infections in Sudanese population.
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