Phytoalexin stilbenoids of Saperavi and Rkatsiteli(Vitis vinifera L.) as biomarkers of resistance to Crown gall infection (Agrobacterium tumefaciens)
DOI:
https://doi.org/10.24203/fq1ykx41Keywords:
Saperavi, Rkatsiteli, Stilbenoids, Phytoalexins, Crown gallAbstract
We have studied the correlation between the phytoalexin stilbenoids synthesis and the level of bacterial and fungal attacks. The paper shows the results of the study related to crown gall infection in different viticulture areas (locations) of Georgia. In concert: for Saperavi - Mukuzani and Napareuli, for Rkatsiteli - Tsarapi and Tibaani. In April 2024 we infected vine trunks with water suspension of “Agrobacterium tumefaciens” under different soil and climatic conditions and we observed the development of diseases in the period of April- October. In experimental vineyards were found out dominant phytoalexin stress- metabolites stilbenoids trans-resveratrol and trans- ɛ -viniferin in the infected vine trunks of Saperavi and Rkatsiteli. Stilbenoids concentration changes were observed under the vine varieties and soil-climatic factors. The impact of mentioned factors were established with different experiments carried out in the lab and in the vineyard. The results are important data for considering the stilbenoids as biomarker of Saperavi and Rkatsiteli vine varieties for their resistance against crown gall disease.
References
[1] Niesen D.aniel, Hessler C. and Navindra S. PBeyond resveratrol: A review of natural stilbenoids identifiedfrom 2009–2013.J.of Berry Research, vol.3,pp.181-196,2013.
[2] Langcake , P., Pryce R. J.The production of resveratrol by Vitis Vinifera and other members of the Vitaceaeas a response to infection or injury. Physiol. Plant Pathol., vol. 9, pp. 77-86, 1976. [3]Langcake P., Cornford C. A., Pryce R. J. Identification of pterostilbene as a phytoalexin from Vitis vinifera leaves. Phytochemistry, vol.18, pp.1025-1027, 1979.
[4] Waterhouse, A. L.,; Lamuela Raventos, R. M.) The occurrence of piceid, a stilbene glucoside in grape ber-ries.Phytochemistry, vol.37, pp. 571-573, 1994.
[5] Langcake , P. Disease resistance of Vitis spp and the production of the stress metabolites resveratrol, E-viniferin, R-viniferin and pterostilbene. Physiol. Plant Pathol.,vol.18,213-226, 1981.
[6] Adrian M., Jeandet P., Bessis R., Joubert J. M. Induction of phytoalexin (resveratrol) synthesis in grapevine leaves treated with aluminum chloride (AlCl3). J. Agric. Food Chem., vol.44:1979-1981, 1996.
[7] Adrian M. Jeandet P., Douillet-Breuil A.C., Tesson L., Bessis R. Stilbene content of mature Vitis vinifera berries in response to UV-C elicitation. J. Agric. Food Chem., vol.48 pp.6103-6105, 2000.
[ 8] Bavaresco, L.,; Petegolli, D.,; Cantu, E.,; Fregoni, M.,; Chiusa , G.,; Trevisan, M., Elicitation and accumulation of stilbene phytoalexins in grapevine berries infected by Botrytis cinerea. Vitis, vol. 36, no.2, pp.77-83, 1997.
[9] Pezet R., Pont V., Mise en e´vidence de pte´rostilbe`ne dans les grappes de Vitis vinifera. Plant Physiol. Biochem., vol.26, pp.603-607, 1988.
[10] Bezhuashvili M.,Bavaresco L., Surguladze M.,Tskhvedadze L., Shoshiashvili G.,Gagunashvili L.,Elanidze L.,Vashakidze P. Stress-metabolite stilbenoids of grape skin of varieties Aleksandrouli and Mujuretuli ( Vitis vinifera L.) in condicion of Gray mold. AGRICULTURE & FOOD, 9th International Conference, August 16-19,Burgas, Bulgar-ia,2021.
[11] Bezhuashvili M., Tskhvedadze L., Surguladze M., Shoshiashvili G., Kharadze Sh., Gagunashvili L., Elanidze L.,Vashakidze P. Stress-metabolitesphytoalexins -stilbenoids of grape skin Rkatsiteli variety (Vitis vinifera L.) in con-dition gray mildew. EurAsian Journal of BioSciences, vol.13, pp.1-6, 2019.
[12] Bezhuashvili M., Tskhvedadze L., Surguladze M., Shoshiashvili G., Elanidze L., Vashakidze P. Impact of trans-Resveratrol on the activity of conidium of the Botrytis cinerea. International Conference onPlant Physiology and Bio-technology, May 06-07, Prague, Czech Republic, 2019.
[13] Adrian M., Jeandet P., Veneau, J., Weston L. A. and Bessis R. Biological activity of resveratrol, a stilbenic com-pound from grapevines, against Botrytis cinerea, the causal agent for gray mold. J. Chem. Ecol., vol.23 no.7, pp.1689-1702, 1997.
[14] Adrian M., Rajaei H., Jeandet P., Veneau J. and Bessis R. Resveratrol Oxidation in Botrytis cinerea Conidia. Phy-topathology, vol.88, no.5, pp.472-476, 1998.
[15] Breuil, A.C., Jeandet, P., Adrian, M., Chopin, F., Pirio, N., Meunier, P. and Bessis R.
Characterization of a Pterostilbene Dehydrodimer Produced by Laccase of Botrytis cinerea.
Phytopathology, vol.89, no.4,pp.298-302, 1999.
[16] Jeandet P., Douillet-Breuil A., Bessis R., Debord S., Sbaghi M., Adrian M., Phytoalexins from the Vitaceae: Bio-synthesis, Phytoalexin Gene Expression in Transgenic Plants, Antifungal Activity, and Metabolism. J. Agric. Food Chem., vol.50, pp.2731−2741, 2002.
[17] Bezhuashvili* M., Bavaresco** L., Surguladze*M., Kharadze* Sh., Shoshiashvili* G., Darchiashvili* N., Vashaki-dze* P. Change of phytoalexin stilbenoids of Vine trunk (Vitis vinifera L.) under Crown gall infection (Agrobacterium tumefaciens).Bulletin of Georgian National Academy of Sciences,vol.18 no.2, pp.133-139, 2024.
[18] Bezhuashvili M., Bavaresco L., Tskhvedadze L., Surguladze M.,Shoshiashvili G., Darchiashvili N., Vashakidze P. Phytoalexin stilbenoids of Saperavi and Rkatsiteli(Vitis vinifera L.) as biomarkers of resistance to gray mold (Botrytis cinerea). Bulletin of Georgian National Academy of Sciences,vol.17,no.2, pp.135-141, 2023.
[19] Bezhuashvili1 M., Bavaresco2 L., Surguladze1 M., Tskhvedadze1 L., Shoshiashvili1 G., Darchiashvili1 N. and Vashakidze1 P. Change of phytoalexin stilbenoids of Vitis vinifera L. cvs. Rkatsiteli and Saperavi under powdery mil-dew infection in the lab and in the vineyard. 45thworld congress of Vine and Wine, Dijon(France), 14-18 october,2024.
[20]. Guebailia H. A., Chira K., Richard T., Mabrouk T. and Furiga A. Hopeaphenol: the first resveratrol tetrameric wines from North Africa. J.Agric. Food Chem., vol.54, pp.9559-9564,2006.
[21] Pastore C., Dal Santo S., Rienth M., Torregrosa L., Luchaire N., Chatbanyong R., Lecourieux D., Kelly M.T., Ro-mieu C. Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (Vitis vi-nifera) fruit. Whole plant temperature manipulation affects flavonoid metabolism and the transcriptome of grapevine berries. Front. Plant Sci; vol.8,pp.929-937, 2017.
[22] Rienth M., Torregrosa L., Luchaire N., Chatbanyong R., Lecourieux D., Kelly M.T., Romieu C. Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (Vitis vinifera) fruit. BMC Plant Biol., vol.14,pp.1–18, 2014.
[23] Wang L., An M., Huang W., Zhan J. Melatonin and phenolics biosynthesis-related genes in Vitis vinifera cell sus-pension cultures are regulated by temperature and copper stress. Plant Cell Tiss. Org.Cult.,vol.138,pp.475–488,2019. [24] Houillé B., Besseau S., Courdavault V., Oudin A., Glévarec G., Delanoue G., Guerin L., Simkin J.A., Papon N., Clastre M., et al Biosynthetic origin of E-resveratrol accumulation in grape canes during postharvest storage. J. Agric. Food Chem., vol.63, pp.1631–1638, 2015.
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Copyright (c) 2025 Marine Bezhuashvili, Luigi Bavaresco, Shorena Kharadze, Magdana Surguladze, Giorgi Shoshiashvili, Nino Darchiashvili, Paata Vashakidze
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