A Review of Microcolorimetry for Textile, Food, Dental and Optoelectronic Industries


  • H. J. Swatland University of Guelph


Microscopy, Fiber optics, Weighted-ordinate colorimetry, Interference colors, Iridescence


A microscope spectrophotometer may be used for colorimetry with transmitted, reflected or emitted light. Optical fibers may be used to link the microscope to remote samples, which may be commercial color standards like Pantone or paint manufacturers’ color chips, or small museum specimens. The weighted-ordinate method of colorimetry gives useful results for a wide variety of samples, although light scattering in small samples like textile fibers has some important effects that may be missed in macroscopic colorimetry. The weighted-ordinate method is applicable to metallic and pastel interference colors, with printed simulations and real interference colors giving similar results. Thus, spectrophotometry is still required to identify fake security holograms.  Some classic work was confirmed, showing how immersion under water may be used to identify a source of iridescence. Multilayer interference in molluscan shell nacre had multiple spectral peaks easily detectable under water, whereas diffractive interference on a peacock feather had a single spectral peak lost under water.  Iridescence in single muscle fibers from roast meat resembled multilayer interference in having multiple spectral peaks that were easily visible under water, and the importance of optical anisotropy in food colorimetry was considered. Boolean analysis of fiber-optic spectra may be used instead of the weighted-ordinate method for spectra obtained robotically in a contextual learning mode. From investigating museum samples to quality control using optoelectronic components, microcolorimetry may have a promising future. 

Author Biography

H. J. Swatland, University of Guelph

University Emeritus Professor


Billmeyer , F.W. , Saltzman, M. (1981). Principles of Color Technology. John Wiley & Sons, New York. 240 pp.

Hooke, R. (1665). Micrographia or Some Physiological Descriptions of Minute Bodies Made by Magnifying Glasses with Observations and Inquiries thereupon. Royal Society, London. Facsimile reproduction, 1961, Dover Publications, New York, p. 168.

Swatland, H.J. (1998). Computer Operation for Microscope Photometry. CRC Press, Boca Raton, FL. 238 pp.

Weidner, V.R., Hsia, J.J. (1981). Reflectance properties of pressed polytetrafluoroethylene powder. J. Optic. Soc. Amer. 71: 856-861. http://hep.ucsb.edu/people/hnn/n/nbspfte.pdf

Swatland, H.J. (1991). Effect of refractive index and cutting angle on internal Fresnel reflectance at the distal window of an optical fiber. J. Comput. Assist. Microsc. 3:233-236.

Greaves, P.H. , Saville, B.P.(1995). Microscopy of Textile Fibres. Royal Microscopical Society, Microscopy Handbooks 32. Bios Scientific Publishers, Oxford. 92 pp.

Ferreira, E.S.B., Hulme, A.N., McNab, H., Quye, A. (2004). The natural constituents of historical textile dyes. Chem. Soc. Rev. 33: 329-336. http://pubs.rsc.org/en/content/articlelanding/2004/cs/b305697j#!divAbstract

Antúnez de Mayolo, K.K. (1989). Peruvian natural dye plants. Econ. Bot. 43: 181-191. https://link.springer.com/article/10.1007/BF02859858

Fischer, C.H., Rabe, J.G., Bischof, M. (1990). Identification of natural and early synthetic textile dyes with HPLC and UV/vis-spectroscopy by diode array detection. J. Liq. Chromatogr. 13: 319-331. http://www.tandfonline.com/doi/abs/10.1080/01483919008049546?needAccess=true&journalCode=ljlc19

Dapson, R. W. (2007). The history, chemistry and modes of action of carmine and related dyes. Biotech. Histochem. 82: 173-187. http://www.tandfonline.com/doi/abs/10.1080/10520290701704188?src=recsys&journalCode=ibih20

Saltzman M. (1992). Identifying dyes in textiles. Amer. Sci. 80: 474-481. http://www.jstor.org/stable/29774728

Gordon, L.M., Cohen, M.J., MacRenaris, K.W., Joester, D. (2015). Dental materials. Amorphous intergranular phases control the properties of rodent tooth enamel. Science 347(6223):746-750. http://science.sciencemag.org/content/347/6223/746

Berthier S. (2007). Iridescences. The Physical Colors of Insects. Springer Science + Business Media: New York; 2007. 160 pp.

Lee, D. (2007). Nature’s Palette. The Science of Plant Color. University of Chicago Press, Chicago. 409 pp.

Hurlbut, C. (1971). Dana’s Manual of Mineralogy. John Wiley & Sons, New York. 579 pp.

Delly, J.G. (2003). The Michel-Lévy interference color chart – microscopy’s magical color key. Modern Microsc. J., July 10. http://www.timeanddate.com/worldclock/city.html?n=1178

Judd, D.B. (1952). Color in Business Science and Industry. John Wiley and Sons: New York. 401 pp.

Kukowski, A.C., Wulf, D.M., Shanks, B.C., Page, J.K., Maddock, R.J. (2004). Factors associated with surface iridescence in fresh beef. Meat Sci. 66: 889-893. http://www.sciencedirect.com/science/article/pii/S0309174003002328

Lawrence, T.E., Hunt, M.C., Kropf, D.H. (2002). Surface roughening of precooked, cured beef round muscles reduced iridescence. J. Muscle Foods 13, 68-73. http://onlinelibrary.wiley.com/doi/10.1111/j.1745-4573.2002.tb00321.x/abstract

Martinez-Hurtado, J.L., Akram, M.H., Yetisen, A.K. (2013). Iridescence in meat caused by surface gratings. Foods 2: 499-506. http://www.oalib.com/paper/3095935#.Wd7AyvkrLDc

Rayleigh, Lord. (1923). Studies of iridescent colour and the structure producing it. III. The colours of Labrador feldspar. Proc. Roy. Soc., London. Series A, 103: 34-45. http://rspa.royalsocietypublishing.org/content/103/720/34

Raman, C.V., Jayaraman, A. (1950). The structure of labradorite and the origin of its iridescence. Proc. Indian Acad. Sci. A32: 1-16. https://link.springer.com/article/10.1007/BF03172469?no-access=true

Bendall, J.R. (1973). Postmortem changes in muscle. In: The Structure and Function of Muscle. G.H. Bourne (ed.). Vol. 2, Part 2. 2nd edition. Academic Press, New York.

Pierobon-Bormioli, S. (1981). Transverse sarcomere filamentous systems: ‘Z- and M-cables’. J. Muscle Res. Cell Motil. 2: 401-413. https://link.springer.com/article/10.1007/BF00711967?no-access=true

Wang, K., Ramirez-Mitchell, R. (1983). A network of transverse and longitudinal intermediate filaments is associated with sarcomeres of adult vertebrate skeletal muscle. J. Cell Biol. 96: 562-570. https://www.ncbi.nlm.nih.gov/pubmed/6682107

Aronson, J.F. (1966). A melting point for the birefringent component of muscle. J. Cell Biol. 30: 453-464. https://www.ncbi.nlm.nih.gov/pubmed/5339378

Swatland, H.J. (1988). Measurement of light scattering in normal pork using a fiber-optic goniophotometer. J. Anim. Sci. 66: 2578-2582. https://www.animalsciencepublications.org/publications/jas/abstracts/66/10/JAN0660102578

Swatland, H.J. (2003). Fiber-optic spectrophotometry of beef relative to sarcomere length. Archiv. Anim. Breeding 46: 31-34. http://www.archanimbreed.com/pdf/2003/at03p031.pdf

Swatland, H.J., Ananthanarayanan, S.P., Goldenberg, A.A. (1994). A review of probes and robots. Implementing new technologies in meat evaluation. J. Anim. Sci. 72: 1475-1486. https://www.animalsciencepublications.org/publications/jas/abstracts/72/6/1475

Swatland, H.J. (1995). Microscope spectrofluorometry of bovine connective tissue using a photodiode array. J. Comput. Assist. Microsc. 7: 165-170.

Lockyer, , J.N. (1883). Studies in Spectrum Analysis. Kegan Paul, Trench & Co. London., 258 pp.

Rood, O.N. (1883). Modern Chromatics with Applications to Art and Industry.2nd edition. Kegan Paul, Trench & Co., London. 330 pp.




How to Cite

Swatland, H. J. (2017). A Review of Microcolorimetry for Textile, Food, Dental and Optoelectronic Industries. Asian Journal of Engineering and Technology, 5(5). Retrieved from https://ajouronline.com/index.php/AJET/article/view/5065