TY - JOUR AU - Rasheed, Ubaid AU - Momin, Faiza AU - Ullah, Hikmat AU - Ali, Imad AU - Nawaz, Haq AU - Ahmad, Latif AU - Khan, Abid AU - Afridi, Mahideen PY - 2021/11/04 Y2 - 2024/03/29 TI - Anti-microbial Activities of Pseudomonas Metabolites Methanolic Extracts against Different MDR Bacterial Pathogens JF - Asian Journal of Applied Sciences JA - AJAS VL - 9 IS - 5 SE - Articles DO - 10.24203/ajas.v9i5.6718 UR - https://ajouronline.com/index.php/AJAS/article/view/6718 SP - AB - <p>Background: A Multiple drug resistance (MDR) in pathogenic bacteria has become a significant public health issue for treatment. Among bacteria, <em>Pseudomonas </em>is another important genus except <em>Streptomyces and Bacillus </em>in production of antimicrobial compounds. The current study aim to to determine the antibacterial activity and preliminary characterization of antibacterial compounds produced by <em>Pseudomonas </em>species such as <em>BB1D11, BN2D41, TG1D11, TR1D41, LH1D11 and TN1D41. </em></p><p>Method: The antibacterial activity was checked by using bio autography method as well as agar well diffusion method, against four multiple drug resistant bacteria including three Gram negative bacteria (<em>E.coli, Acinetobacter </em>and <em>Pseudomonas</em>) and one Gram positive bacterium (Methicillin Resistant <em>Staphylococcus aureus</em>). Isolation test showed good activity against all the four MDR bacteria, by producing clear zone of diameter from 2mm up to 20mm. Optimum temperature for growth and antibiotic production of <em>Pseudomonas </em><em>BB1D11, BN2D41, TG1D11, TR1D41, LH1D11 and TN1D41</em> was 37C<sup>0</sup>. It produces more metabolites when subjected to shaking incubation. In thin layer chromatography, the extracts were repeatedly inserted on a silica gel coated plate, which was run in mobile phase. Normal HPLC was perform to reveal the presence of antibacterial compounds.</p><p>Results:  By well diffusion assay a zone of inhibition range from 2-18 mm of diameter against different test bacteria. The components were separated, resulting in the formation of bands with different colors, each showing a different compounds. Biological screening was performed by bio autography, metabolites showed a significant activity at retention factor of 0.89. While HPLC at retention time 2.50-2.90 showed presence of significant antibacterial compounds.</p><p>Conclusion:  <em>Pseudomonas </em><em>BB1D11, BN2D41, TG1D11, TR1D41, LH1D11 and TN1D41</em> showed promising anti-microbial activity against different MDR bacteria. It is concluded that HPLC revealed the presence of DAPG at retention time 2.90 which inhibit the growth of MDR bacterial strains.</p> ER -