TY - JOUR AU - Arsan, Akhmad Gazali AU - Ilhamiyah, Achmad Jaelani, PY - 2015/06/24 Y2 - 2024/03/29 TI - Patogenicity of Bacillus thuringiensis which Isolated from Tidal Ecosystem against Diamond Backmoth Larvae, Plutella xylostella Linn JF - Asian Journal of Applied Sciences JA - AJAS VL - 3 IS - 3 SE - Articles DO - UR - https://ajouronline.com/index.php/AJAS/article/view/2721 SP - AB - <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>EN-US</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:DontVertAlignCellWithSp /> <w:DontBreakConstrainedForcedTables /> <w:DontVertAlignInTxbx /> <w:Word11KerningPairs /> <w:CachedColBalance /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> <m:mathPr> <m:mathFont m:val="Cambria Math" /> <m:brkBin m:val="before" /> <m:brkBinSub m:val="&#45;-" /> <m:smallFrac m:val="off" /> <m:dispDef /> <m:lMargin m:val="0" /> <m:rMargin m:val="0" /> <m:defJc m:val="centerGroup" /> <m:wrapIndent m:val="1440" /> <m:intLim m:val="subSup" /> <m:naryLim m:val="undOvr" /> </m:mathPr></w:WordDocument> </xml><![endif]--> <p class="MsoNormal" style="text-align: justify;"><span class="hps"><span style="mso-bidi-font-weight: normal;"><span style="mso-bidi-font-style: normal;"><span style="mso-ansi-language: EN;" lang="EN">The purpose</span></span></span></span><span style="mso-bidi-font-weight: normal;"><span style="mso-bidi-font-style: normal;"><span style="mso-ansi-language: EN;" lang="EN"> <span class="hps">of </span><span style="mso-spacerun: yes;"> </span><span class="hps">this research</span> <span class="hps">was</span> <span class="hps">1)</span> <span class="hps">the exploration</span> <span class="hps">of Bacillus thuringiensis</span> <span class="hps">in</span> <span class="hps">soil</span>, <span class="hps">water</span>, <span class="hps">and</span> <span class="hps">organic matter</span> <span class="hps">contained in the</span> <span class="hps">tidal area</span> <span class="hps">of the</span> <span class="hps">South</span> <span class="hps">Kalimantan</span> <span class="hps">and</span> <span class="hps">Central Kalimantan</span>; <span class="hps">2</span>) <span class="hps">Comparing the</span> <span class="hps">pathogenicity</span> <span class="hps">among</span> <span class="hps">isolates</span> <span class="hps">of B. thuringiensis</span> <span class="hps">were</span> <span class="hps">found against</span> <span class="hps">Plutella</span> <span class="hps">xylostella</span> <span class="hps">larvae</span>; <span class="hps">3</span>) <span class="hps">To test the</span> <span class="hps">effectiveness of the</span> <span class="hps">highest</span> <span class="hps">pathogenicity</span> <span class="hps">B.</span> <span class="hps">thuringiensis</span> <span class="hps">isolates</span> <span class="hps">from</span> <span class="hps">isolates</span> of <span class="hps">exploration results</span> <span class="hps">against</span> <span class="hps">P.</span> <span class="hps">xylostella</span> <span class="hps">larvae</span>. <span class="hps">Soil samples</span> <span class="hps">taken</span> <span class="hps">from</span> <span class="hps">six areas</span> <span class="hps">of land</span> <span class="hps">in tidal area of</span> <span class="hps">the Barito Kuala</span>, <span class="hps">Banjarmasin</span>, <span class="hps">Banjar district,<span style="mso-spacerun: yes;"> </span>Province South</span> <span class="hps">Kalimantan</span>, Pulang Pisau <span class="hps">and</span> <span class="hps">Kapuas of</span> <span class="hps">Central</span> <span class="hps">Kalimantan Province</span>. <span class="hps">Isolation</span> <span class="hps">of bacteria</span> <span class="hps">and</span> <span class="hps">pathogenicity</span> <span class="hps">tests</span> <span class="hps">conducted</span> <span class="hps">in the</span> <span class="hps">the laboratory</span> <span class="hps">of Plant</span> <span class="hps">Pests and Diseases</span> <span class="hps">Department of</span> <span class="hps">the Faculty of</span> <span class="hps">Agriculture, Lambung Mangkurat University, Banjarbaru</span>. <span class="hps">Research</span> <span class="hps">carried out</span> <span class="hps">for 8</span> <span class="hps">months</span>. <span class="hps">Pathogenicity</span> <span class="hps">carried out</span> <span class="hps">by determining the</span> <span class="hps">level of</span> <span class="hps">LC<sub>50</sub></span> <span class="hps">value</span> <span class="hps">from</span> <span class="hps">each</span> <span class="hps">B.</span> <span class="hps">thuringiensis</span> <span class="hps">were found</span> <span class="hps">by</span> <span class="hps">probit</span> <span class="hps">analysis</span>. <span class="hps">Efficacy Test of</span> <span class="hps">B.</span> <span class="hps">thuringiensis</span> <span class="hps">implemented using</span> <span class="hps">completely randomized design</span>, <span class="hps">which</span> <span class="hps">consists</span> <span class="hps">of</span> <span class="hps">5</span> <span class="hps">treatments</span> <span class="hps">and</span> <span class="hps">4</span> <span class="hps">replicates</span> <span class="hps">ie</span> <span class="hps">a)</span> <span class="hps">2</span> <span class="hps">cc</span> <span class="hps">suspension</span> <span class="hps">of<span style="mso-spacerun: yes;"> </span>Bacillus thuringiensis/</span> <span class="hps">l water</span>; <span class="hps">b</span>) <span class="hps">3</span> <span class="hps">cc</span> <span class="hps">suspension</span> <span class="hps">of Bacillus thuringiensis/l water</span>; <span class="hps">c</span>) <span class="hps">4</span> <span class="hps">cc</span> <span class="hps">suspension</span> <span class="hps">of Bacillus thuringiensis/</span> <span class="hps">l water</span>; <span class="hps">d</span>) <span class="hps">Application</span> <span class="hps">of <span style="mso-spacerun: yes;"> </span>klorfluazuron</span> <span class="hps">insecticide,<span style="mso-spacerun: yes;"> </span>the</span> <span class="hps">trade name</span> <span class="hps">Atabron</span> <span class="hps">with</span> <span class="hps">a concentration of 2</span> <span class="hps">cc</span> <span class="hps">/</span> <span class="hps">l water</span>; <span class="hps">e</span>) <span class="hps">A</span>pplied <span class="hps">with</span> <span class="hps">water</span>. <span style="mso-spacerun: yes;"> </span><span class="hps">Differences</span> <span class="hps">between</span> <span class="hps">treatment</span> <span class="hps">effect</span> <span class="hps">is determined</span> <span class="hps">by</span> <span class="hps">Duncan's Multiple Range</span> <span class="hps">Test</span> <span class="hps">(</span>DMRT). <span class="hps">The final conclusion is</span> <span class="hps">that</span>: <span class="hps">a)</span> <span class="hps">Results of exploration was found</span> <span class="hps">11</span> <span class="hps">(</span>eleven) <span class="hps">isolates of</span> <span class="hps">B.</span> <span class="hps">thuringiensis</span> <span class="hps">in the</span> <span class="hps">areas</span> of <span class="hps">tidal</span> <span class="hps">ecosystems</span>; <span class="hps">b</span>) <span class="hps">The higher</span> <span class="hps">the concentration of</span> <span class="hps">cells</span> <span class="hps">of B. thuringiensis</span> <span class="hps">more</span> <span class="hps">larvae</span> <span class="hps">of <span style="mso-spacerun: yes;"> </span>P.</span> <span class="hps">xylostella</span> <span class="hps">were</span> <span class="hps">dead</span>; <span class="hps">b</span>) <span class="hps">the</span> <span class="hps">high</span> <span class="hps">pathogenicity</span> <span class="hps">of <span style="mso-spacerun: yes;"> </span>B. thuringiensis</span> <span class="hps">isolates</span> <span class="hps">obtained</span> <span class="hps">on</span> <span class="hps">isolated</span> <span class="hps">from</span> <span class="hps">drains ecosystems</span> <span class="hps">(</span>sewers) <span class="hps">on</span> <span class="hps">forest</span> <span class="hps">with</span> <span class="hps">LC<sub>50</sub> values of</span> <span class="hps">2.41</span> <span class="hps">x</span> <span class="hps">10<sup>7</sup></span><sup> </sup><span class="hps">cells</span> <span class="hps">/</span> <span class="hps">ml</span> <span class="hps">of water</span>; <span class="hps">d</span>) <span class="hps">The concentration of</span> <span class="hps">B.</span> <span class="hps">thuringiensis</span> <span class="hps">the most</span> <span class="hps">effective</span> <span class="hps">in reducing</span> <span class="hps">leaf</span> <span class="hps">damage</span> <span class="hps">is</span> <span class="hps">4</span> <span class="hps">cc</span> <span class="hps">/</span> <span class="hps">l</span>.</span></span></span></p> <p class="MsoNormal" style="text-align: justify;"><span style="mso-bidi-font-weight: normal;"><span style="mso-ansi-language: EN;" lang="EN"> </span></span></p> <div style="mso-element: para-border-div; border: none; border-bottom: solid windowtext 1.0pt; mso-border-bottom-alt: solid windowtext .75pt; padding: 0cm 0cm 1.0pt 0cm; margin-left: 0cm; margin-right: -.35pt;"><p class="MsoNormal" 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