@article{Budiawan_Dani_Saefumillah_Handayani_2016, title={The Formation of 8-OHDG from Toxic Substances Trigger to Free Radicals}, volume={4}, url={https://ajouronline.com/index.php/AJAS/article/view/3539}, abstractNote={<p>This study is conducted to see the profile of DNA <em>Adduct</em> (8-OHdG) formation as DNA damage indicators, by using <em>calf thymus</em> DNA incubated with toxic and carcinogenic compounds. The compounds which could trigger free radicals in this research are PAH(Benzo[a]Pyrene), and CuCl<sub>2</sub>. <em>Calf thymus</em> DNA was incubated with Benzo[a]Pyrene and CuCl<sub>2</sub> compounds under pH and temperature variations to induce the formation of reactive oxygen species (ROS) in the process of oxidative DNA damage. From this research, all of compounds have potency to trigger the formation of DNA <em>Adduct</em> (8-OHdG). The ratio of absorbance to assess the purity of DNA at 260 nm and 280 nm (λ260/ λ280)  is measured at ~1.9. The shifted peaks at λmax are indicating changes on structures of DNA as a result of <em>calf thymus</em> DNA incubation with B[a]P and CuCl<sub>2</sub>. The highest level of 8-OHdG results in <em>calf thymus</em> DNA incubation with B[a]P and CuCl<sub>2</sub> under pH 8.5 and incubation temperature at 60°C, is about 120.856 μg/L.</p><p> </p>}, number={1}, journal={Asian Journal of Applied Sciences}, author={Budiawan, R. and Dani, Intan Cahaya and Saefumillah, Asep and Handayani, Sri}, year={2016}, month={Feb.} }