Sensitivity Test and Specificity Invention Gram Negative Rods with Gram Staining for Screening Test of Urinary Tract Infection (UTI)

Authors

  • Siti Zaetun Polytechnic of Health Ministry of Health Mataram
  • Lalu Sri Gede
  • Ms. Agrijanti
  • Ayu Anulus
  • Kadeq Novita Prajawanti

Keywords:

Gram Staining, Sensitivity, Specificity, Urinary Tract Infection (UTI)

Abstract

Screening test is not for diagnose, but to determine whether the one who involved in this ailment sick or not. Then for those with positive result will be threating intensively. In order to prevent the infection. Because screening test is not for diagnose, there for it is requires direct follow up and appropriate treatment. The formulation of this research was the sensitivity and specificity’s values of gram negative rods invention with Gram staining as a screening test of urinary tract infection (UTI). This research was a cross sectional study, the samples of this research was 18-20 years old students of laboratory technician program in Polytechnic of Health Ministry of Health Mataram taken with consecutive sampling method. The urine culture as a Gold standard of urinary tract infection (UTI) test. The result from this research shows that 8 samples with bacteria positive and 69 samples with negative result from 77 samples. The Gram staining from samples shows that 9 samples with positive result and 76 are negative. Urine microscopy test show 15 samples are positive leukocyturia and 62 are negative. Sensitivity and specificity value present from the 2 x 2 chi – square table. Sensitivity value is 75.05% and specificity is 80,51%. Gram staining of urine without centrifugation result is higher than leukocyturiaurine. 

References

Arikunto, Suharsimi, â€Research An Approach Procedures Practicalâ€, Ed.14. Jakarta: Rineka Cipta, 2010.

Arslan, S., Caksen, H., Rastgeldi, L., et al., â€Use of urinary Gram stain for detection of urinary tract infection in childhoodâ€, Yale J of Biol and Med, 75, 73-8, 2002.

Beyene, G., Tsegaye, W, â€Bacterial uropathogens in urinary tract infection and antibiotic susceptibility pattern in jimma university specialized hospital, southwest ethiopiaâ€, Ethiop J Health Sci, Vol. 21, No. 2, pp. 141-6, 2011.

Bjerklund Johansen TE, et al., â€Prevalence of hospital-acquired urinary tract infections in urology departmentsâ€, Eur Urol, Vol. 51, No. 4, pp. 1100-11, 2007.

Christenson RH, Tucker JA, Allen E., â€Results of Dipstick Tests, Visual Inspection, Microscopic Examination of Urine Sediment, and Microbiological Cultures of Urine Compared for Simplifying Urinalysisâ€, Clin Chem, Vol. 31, pp. 448-50, 1985.

Enggram, Barbara, â€Nursing care plan medical surgeryâ€, Vol. 3, Jakarta: EGC, 1998.

Foxman B, â€Epidemiology of urinary tract infections: incidence, morbidity, and economic costsâ€, Am J Med, Vol. 113, pp. 5-13, 2002.

Grude, N., Tveten, Y., & Kristiansen B. E., â€Urinary tract infections in Norway: bacterial etiology and susceptibility, a retrospective study of clinical isolatesâ€, Clin Microbiol Infect, Vol. 7, pp. 543-7, 2001.

Korrapati Narasimhulu, P. Sreenivasa Rao and A.Venu Vinod, â€Isolation and Identification of Bacterial Strains and Study of their Resistance to Heavy Metals and Antibioticsâ€, Journal Microbial Biochem Technol, Vol. 2, 2010

Maki DG, et al., â€Engineering out the risk for infection with urinary cathetersâ€, Emerg Infect Dis, Vol.7, No. 2, pp. 342-7, 2001.

Mazzulli T, â€Resistance trends in urinary tract pathogens and impact on managementâ€, J Urol, 2002.

Morgan, Robert M. and Shelby D. Hunt, â€The Commitment Trust Theory of Relationship Marketing. Journal of Marketingâ€, Vol. 58, No. 3,1994.

Nugroho, Wahyudi, â€Communication in Nursing Gerontikâ€, .Jakarta: EGC, 2009.

Ruden H, et al., â€Nosocomial and community-acquired infections in Germanyâ€, Summary of the results of the First National Prevalence Study (NIDEP), Vol. 25, No. 4, pp. 199-202, 1997.

Rushton, H. G, â€Urinary Tract Infections in Childrenâ€, Pediatric Clinics of North America, Vol. 44, pp.1133-1169, 1997.

Ryu KH, Kim MK and Jeong YB, â€A Recent Study on the Antimicrobial Sensitivity of the Organisms that Cause Urinary Tract Infectionâ€, Korean J Urol, Vol. 48, No. 6, pp. 638-645, 2007.

Schneider, P.F., & Riley T. V, â€Staphylococcus saprophyticus urinary tract infections: Epidemiological data from Western Australiaâ€, Eur J Epidemiol, Vol. 12, pp. 51-54, 1996.

Sukandar, Enday, â€Nephrology Clinic†Ed. 3, Bandung: Publisher PPI part of Medicine RSH, 2006.

UVI , â€nedre urinvägsinfektioner hos kvinnor [UTI - lower urinary tract infections in females]â€, The Medical Products Agency, Sweden, 2007.

Tambyah P, et al., â€Urinary catheters and drainage systems: definition, epidemiology and risk factorsâ€, In Urogenital Infections, Naber KG, et al., Editors. European Association of Urology: Arnhem, The Netherlands, pp. 523-31, 2010.

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Published

2017-02-20

How to Cite

Zaetun, S., Gede, L. S., Agrijanti, M., Anulus, A., & Prajawanti, K. N. (2017). Sensitivity Test and Specificity Invention Gram Negative Rods with Gram Staining for Screening Test of Urinary Tract Infection (UTI). Asian Journal of Applied Sciences, 5(1). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/4361