Distribution and Characterization of Actinomycetes in Suez Bay Sediments, Egypt

Authors

  • Hanan M. Abd-Elnaby
  • Gehan M. Abo-Elala
  • Usama M. Abdel-Raouf
  • Moaz M. Hamed

Keywords:

actionomycetes, Streptomyces parvus, Streptomyces griseorubens, Kocuria palustris, antibacterial

Abstract

Sediment samples were collected seasonally (from winter to autumn 2012) from ten sampling sits along the sea shore of Suez Bay for the purpose of studying the distribution of actinomycetes and its relation with the physicochemical parameters. Multiple regression analysis was applied to reflect the relationship between the actinomycetes counts and the environmental factors of sediments during the different seasons. Seventeen actinomycetes isolates were selected to represent all colony morphologies observed on the starch nitrate agar plates. Pure isolates were then subjected to morphological, physiological and biochemical characterization (phenotypic characterization). Phenotypic characters were coded in a binary form of the presence/absence type. Analysis of the results by numerical techniques using the simple matching coefficient (SSM) and the unweighted pair group method with arithmetic mean (UPGMA) grouped the isolates into two main clusters, cluster A (3 isolates) and cluster B (11 isolates), in addition to single clustered isolates (3 isolates). Seven out of the seventeen isolates (40% of the isolates) exhibited antimicrobial activities against different pathogenic indicators. Genotypic characterizations of the most potent isolates were performed using 16S sequence analysis, where the isolates under study were identified as Streptomyces parvus, Streptomyces griseorubens (cluster A) and Kocuria palustris (cluster B).

Author Biographies

  • Hanan M. Abd-Elnaby
  • Gehan M. Abo-Elala
  • Usama M. Abdel-Raouf
  • Moaz M. Hamed

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Distribution and Characterization of Actinomycetes in Suez Bay Sediments, Egypt. (2016). Asian Journal of Applied Sciences, 4(1). https://ajouronline.com/index.php/AJAS/article/view/3596

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