A Beta-glucosidase with Beta-xylosidase Activity from the Digestive Juice of the Land Crab <i>Cardisoma armatum</i>


  • Claude K. Ya
  • Fankroma M. T. Koné University Nangui Abrogoua, Laboratory of Biocatalysis and Bioprocessing
  • Sophie N. Gnangui
  • Soumaila Dabonné
  • Lucien P. Kouamé


Bifunctional enzyme, β-glucosidase, β-xylosidase, land crab, Cardisoma armatum, transglycosylation


A bifunctional beta-glucosidase/beta-xylosidase enzyme from land crab Cardisoma armatum was purified to homogeneity by anion-exchange, size-exclusion, cation-exchange and hydrophobic interaction chromatographies and characterized to explore his suitability in biotechnology and to elucidate his role in biodegradation of plant material. The enzyme showed a single protein band and its relative molecular weight was estimated to be 69.81 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 115.24 kDa by gel filtration, indicating that the enzyme should be monomeric or homo-oligomeric enzyme. The specific activities towards para-nitrophenyl-beta-D-glucopyranoside and para-nitrophenyl-beta-D-xylopyranoside were 74.16 ± 2.10-4 and 88.60 ± 2.10-3 U/mg of protein, respectively. This enzyme was optimally active at pH 5.6 and at 60 °C, and was stable at pH range 4.6 to 6.6. The only substrates that were hydrolyzed by the purified enzyme were para-nitrophenyl-beta-D-glucopyranoside, para-nitrophenyl-beta-D-xylopyranoside, xylobiose, cellobiose and cellodextrins. The enzyme was specific for substrates with a beta-(1,4)-glucosidic linkages and was an exo-glycosidase. The catalytic efficiencies values for beta-D-1,4-glucoside and beta-D-1,4-xyloside were respectively 1666.67 and 132.89 mM-1s-1. The purified enzyme is a beta-glucosidase with beta-xylosidase activity, which takes effect after actions of endo-enzymes and exo-enzymes and it hydrolyzes cellobiose and xylobiose in glucose and xylose, respectively. However, the beta-glucosidase/beta-xylosidase catalyzed both hydrolysis and transglycosylation.



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How to Cite

Ya, C. K., Koné, F. M. T., Gnangui, S. N., Dabonné, S., & Kouamé, L. P. (2014). A Beta-glucosidase with Beta-xylosidase Activity from the Digestive Juice of the Land Crab <i>Cardisoma armatum</i>. Asian Journal of Applied Sciences, 2(4). Retrieved from https://ajouronline.com/index.php/AJAS/article/view/1444




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