@article{Muradas_Weiss_Kozicki_Granemann_Moreira_Souza_Santos_Abreu_Green_Milleo_2013, title={The Viability of Cryopreserved Spermatozoa Recovered From The Cauda Epididymis of Equines and The Time of Collection Post-Orchiectomy}, volume={1}, url={https://ajouronline.com/index.php/AJAFS/article/view/8}, abstractNote={<span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">The experiment was conducted using 20</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB"> epididymides recovered from ten stallions with the objective of correlating</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB"> the viability of </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">cryopreserved</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB"> spermatozoa derived</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB"> from the cauda epididymis of these animals and the time of collection of the spermatozoa post-orchiectomy.</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">After submitting the stallions to bilateral orchiectomy, the</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB"> spermatozoa were flushed from each cauda</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB"> epididymis and subsequently cryopreserved for further evaluation. The </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB">spermatozoa were classified into two groups (G)</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">: G1- <span style="font-weight: normal;">cryopreserved ejaculated spermatozoa</span></span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB"> (</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">pre-orchiectomy); and G2- cryopreserved </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB">spermatozoa</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB"> recovered from the cauda epididymis (post-orchiectomy). The</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 8.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB"> following parameters were evaluated</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB">, using the double stain</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB"> Tripan-blue/Giemsa to determine</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 8.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">: defects in the acrosome</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB"> and the number of live and dead spermatozoa. </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">The results obtained demonstrated that the cryopreserved sperm from ejaculated semen samples corresponded to 65% of live </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB">spermatozoa</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB"> with acrosomes. This was significantly higher (p<0,01) when compared to cryopreserved </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB">spermatozoa</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB"> recovered from the cauda epididymis (45%), zero hours post-orchiectomy. The rate of live</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB"> spermatozoa </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">with acrosomes declined rapidly 24-hours post-orchiectomy in relation to prior periods. Thirty-six (36) hours post-orchiectomy the rate of live </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 9.5pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: PT-BR; mso-bidi-language: AR-SA;" lang="EN-GB">spermatozoa</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB"> was significantly lower (p <0.05) than at 24 hours, but represented 17% of live sperm cells with acrosomes. This study demonstrates that it is possible to recover viable spermatic cells and freezing them 36 hours post-orchiectomy. Therefore, the data suggests that it is possible to transfer the methodology employed in this study to be utilized in </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">genetically valuable </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 10pt; mso-bidi-font-size: 12.0pt; mso-fareast-font-family: ’Times New Roman’; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;" lang="EN-GB">stallions in the event of sudden death so as to conserve their semen for future use.</span>}, number={1}, journal={Asian Journal of Agriculture and Food Sciences}, author={Muradas, Priscilla Ricabone and Weiss, Romildo Romualdo and Kozicki, Luiz Ernandes and Granemann, Luiz Carlos and Moreira, Ney and Souza, Jose and Santos, Ivo Walter and Abreu, Renata Azevedo and Green, Kenny and Milleo, Thalitta Capalbo}, year={2013}, month={Apr.} }