The Viability of Cryopreserved Spermatozoa Recovered From The Cauda Epididymis of Equines and The Time of Collection Post-Orchiectomy


  • Priscilla Ricabone Muradas Evangelica Faculty of Veterinary Medicine- Curitiba
  • Romildo Romualdo Weiss University Federal of Parana
  • Luiz Ernandes Kozicki Pontifical Catholic University of Parana
  • Luiz Carlos Granemann Autonomous Veterinary
  • Ney Moreira Federal University of Paran
  • Jose Souza Veterinary autonomous
  • Ivo Walter Santos Department of Veterinary Medicine
  • Renata Azevedo Abreu Biotechnological Processes of UFPR
  • Kenny Green Student at Federal university of Parana
  • Thalitta Capalbo Milleo Master of Science at Pontifical Catholic University of Parana


cryopreservation, epididymis, spermatozoa, stallion, trypan blue/giemsa


The experiment was conducted using 20 epididymides recovered from ten stallions with the objective of correlating the viability of cryopreserved spermatozoa derived from the cauda epididymis of these animals and the time of collection of the spermatozoa post-orchiectomy.After submitting the stallions to bilateral orchiectomy, the spermatozoa were flushed from each cauda epididymis and subsequently cryopreserved for further evaluation. The spermatozoa were classified into two groups (G): G1- cryopreserved ejaculated spermatozoa (pre-orchiectomy); and G2- cryopreserved spermatozoa recovered from the cauda epididymis (post-orchiectomy). The following parameters were evaluated, using the double stain Tripan-blue/Giemsa to determine: defects in the acrosome and the number of live and dead spermatozoa. The results obtained demonstrated that the cryopreserved sperm from ejaculated semen samples corresponded to 65% of live spermatozoa with acrosomes. This was significantly higher (p<0,01) when compared to cryopreserved spermatozoa recovered from the cauda epididymis (45%), zero hours post-orchiectomy. The rate of live spermatozoa with acrosomes declined rapidly 24-hours post-orchiectomy in relation to prior periods. Thirty-six (36) hours post-orchiectomy the rate of live spermatozoa was significantly lower (p <0.05) than at 24 hours, but represented 17% of live sperm cells with acrosomes. This study demonstrates that it is possible to recover viable spermatic cells and freezing them 36 hours post-orchiectomy. Therefore, the data suggests that it is possible to transfer the methodology employed in this study to be utilized in genetically valuable stallions in the event of sudden death so as to conserve their semen for future use.

Author Biographies

Romildo Romualdo Weiss, University Federal of Parana

Department of Veterinary Medicine

Luiz Ernandes Kozicki, Pontifical Catholic University of Parana

School of Agricultural Science and Veterinary Medicine

Ney Moreira, Federal University of Paran

Department of Veterinary Medicine

Renata Azevedo Abreu, Biotechnological Processes of UFPR

Doctorate at Federal University of Parana

Kenny Green, Student at Federal university of Parana

Departament of Veterinary Medicine

Thalitta Capalbo Milleo, Master of Science at Pontifical Catholic University of Parana

Master of Science at Pontifical Catholic University of Parana


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How to Cite

Muradas, P. R., Weiss, R. R., Kozicki, L. E., Granemann, L. C., Moreira, N., Souza, J., Santos, I. W., Abreu, R. A., Green, K., & Milleo, T. C. (2013). The Viability of Cryopreserved Spermatozoa Recovered From The Cauda Epididymis of Equines and The Time of Collection Post-Orchiectomy. Asian Journal of Agriculture and Food Sciences, 1(1). Retrieved from




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