The Viability of Cryopreserved Spermatozoa Recovered From The Cauda Epididymis of Equines and The Time of Collection Post-Orchiectomy
Keywords:cryopreservation, epididymis, spermatozoa, stallion, trypan blue/giemsa
AbstractThe experiment was conducted using 20 epididymides recovered from ten stallions with the objective of correlating the viability of cryopreserved spermatozoa derived from the cauda epididymis of these animals and the time of collection of the spermatozoa post-orchiectomy.After submitting the stallions to bilateral orchiectomy, the spermatozoa were flushed from each cauda epididymis and subsequently cryopreserved for further evaluation. The spermatozoa were classified into two groups (G): G1- cryopreserved ejaculated spermatozoa (pre-orchiectomy); and G2- cryopreserved spermatozoa recovered from the cauda epididymis (post-orchiectomy). The following parameters were evaluated, using the double stain Tripan-blue/Giemsa to determine: defects in the acrosome and the number of live and dead spermatozoa. The results obtained demonstrated that the cryopreserved sperm from ejaculated semen samples corresponded to 65% of live spermatozoa with acrosomes. This was significantly higher (p<0,01) when compared to cryopreserved spermatozoa recovered from the cauda epididymis (45%), zero hours post-orchiectomy. The rate of live spermatozoa with acrosomes declined rapidly 24-hours post-orchiectomy in relation to prior periods. Thirty-six (36) hours post-orchiectomy the rate of live spermatozoa was significantly lower (p <0.05) than at 24 hours, but represented 17% of live sperm cells with acrosomes. This study demonstrates that it is possible to recover viable spermatic cells and freezing them 36 hours post-orchiectomy. Therefore, the data suggests that it is possible to transfer the methodology employed in this study to be utilized in genetically valuable stallions in the event of sudden death so as to conserve their semen for future use.
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