The Pathogenicity of Beauveria bassiana on Diatraea centrella Larva of Sugarcane in Guyana

Basantie Sukhu, Gomathinayagam Subramanian, Abdullah Ansari


Sugarcane is a highly beneficial crop grown worldwide and is greatly influenced by numerous insect pests. These insect pests have adapted to the diversity of conditions under which sugarcane is grown thus causing a great degree of damage to the crop. These insect pests feed on different parts of the sugarcane. For instance, the early shoot borer attacks the cane before internode formation, while the internode borer feeds after internode formation, the foliar pest feeds on the leaves and some would feed on the rootstalks.

Guyana has large areas of land which are free of agricultural chemicals and thus are suitable for organic farming. Organic farming is practiced in Guyana on a small scale. However, crops such as cocoa, pineapple and heart of palm have already been grown organically for export. Similarly, like organic farming, biological control of pests and diseases is also encouraged worldwide. Biological control in agriculture is a method of controlling pests such as insects, weeds and diseases by using natural enemies such as predators, parasites, herbivores, or any other natural mechanisms. In addition, like organic farming biological controls are also ecologically friendly and are very effective. Both approaches (organic farming and biological control) are significant since the use of pesticides and chemical fertilizers would be eliminated, thus reducing health issues in the environment and of consumers.

                This research work is on the pathogenicity of Beauveria bassiana on the insect pest Diatraea centrella larva of sugarcane in Guyana. The entomopathogenic fungus, Beauveria bassiana, is a promising control agent against many insect pests [1]. Beauveria bassiana is commonly found in soils worldwide and has a host range of over 700 insect species, including insects from the orders Lepidoptera, Coleoptera and Hymenoptera.  The research carried out focuses on the effect of Beauveria bassiana spores on Diatraea centrella larva and the rate at which these spores are effective, as well as the target site of infection of these spores on the larva.

               The study conclusively proves that a higher number of Beauveria bassiana spores are more effective on Diatraea centrella larva in a short period of time and the dorsal, ventral and intestinal tissues of the Diatraea centrella larva are more susceptible to the spores of Beauveria bassiana. 


Pathogenicity, Beauveria bassiana, Diatraea centrella, Sugarcane, Pathogenicity

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McCoy, C.W. 1990. Entomogenous fungi as microbial pesticides. Pages 139-159 in R.R. Baker and P.E. Dunn (eds.), New Direction in Biological Control. A.R. Liss, New York.

Davis, H.B., Eastwood, D., Stuart, W. L. M. and Surujbally, N. 2004. The Sugar Industry in Guyana. In Sugar Cane International, 22(4): 3-10.

Luiz Augusto Horta, 2007. Bio fuel potential in Guyana. Economic Commission for Latin America and the Caribbean (ECLAC). P. 11-19.

Bates, J. F.1954. The status of moth borer in British Guiana. Proc. Br. W. Indies Sug. Technol. P. 126-136.

Bates, J. F.1967. Investigations on moth borers in British Guiana. Proc. Int. Soc. Sug. Cane Technol. 12: 1349-1367.

Long, D.W., G.A. Drummond, E. Groden. 2000. Horizontal transmission of Beauveria bassiana. Agriculture and Forest Entomology 2:11-17.

Goettel, M. S., Inglis, G. D. and Wraight, S. P. 2000. Fungi. In Field Manual of Techniques in Invertebrate Pathology. (Eds. Lacey and Kaya. Kluwer) Academic Press. P. 255-282.

Wraight, S.P. and Ramos, M. E. 2002. Application factors affecting the field efficacy of Beauvaria bassiana foliar treatments against the Colorado potato beetle Leptinotarsa decemlineata. Biological Control. 23(2):164-178.

Achonduh, O. A. and Tondje, P. R. 2008. First report of pathogenicity of Beauveria bassiana RBL1034 to the malaria vector, Anopheles gambiae.. (Diptera; Culicidae) in Cameroon. African Journal of Biotechnology, 7 (8):931-935.

Tafoya, F., Zuñiga -Delgadillo, M., Alatorre, R., Cibrian-Tovar, J. and Stanley, D. 2004. Pathogenicity of Beauveria bassiana (Deuteromycota: hyphomycetes) against the cactus weevil, Metamasius spinolae (Coleoptera: curculionidae) under laboratory conditions. Entomology Department, University of Nebraska-Lincoln. Florida Entomologist. Vol. 87(4) 533-536.

Padmaja, V. and Kaur, G. 2001. Use of the fungus Beauveria bassiana (Bals.) Vuill (Moniliales: Deuteromycetes) for controlling termites. Department of Botany, Andhra University, Visakhapatnam 530 003, India. Current Science, 81(6):645-647.

Ghose, K. C. and Manna, B. 2007, Practical Zoology, New Central Book Agency (P) Limited, 2007 - Biological Sciences - 481 pages.

Thamarai Chelvi, C., W. Richard Thilagaraj, R. Kandasamy, 2010, Laboratory culture and virulence of Beauveria brongniarti isolates on sugarcane white grub, Holotrichia serrata F(Coleoptera : Scarabidae), Journal of Biopesticides 3: 177 – 179.



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