Isolation and Identification of a Begomovirus Associated With the Leaf Curl Disease of Gumamela (<i>Hibiscus rosa-sinensis</i>) in the Philippines

Authors

  • Lolita Martinez Dolores Crop Science cluster, Institute of plant Breeding, University of the Philippines Los Banos (UPLB)
  • Maricel Gonzales Crop Science Cluster, Institute of Plant Breeding, University of the Philippines Los Banos (UPLB)
  • Pablito Magdalita Crop Science Cluster, Institute of Plant Breeding, University of the Philippines Los Banos (UPLB)

Keywords:

begomovirus, Cotton leaf curl Multan virus, Hibiscus rosa-sinensis, gumamela leaf curl

Abstract

Hibiscus rosa-sinensis, locally known as ‘gumamela’ were observed with virus symptoms of mosaic, leaf curl, cupping, enation, mottling, and stunting. The virus was transmitted by whiteflies and by grafting to different host plant species including: Gossypium hirsutum, Vigna unguiculata and Hibiscus rosa sinensis but not to Abelmoschus esculentus, Datura metel, D. stramonium, Solanum melongena , Lycopersicon esculentum, Nicotiana glutinosa and  Physalis floridana . The thirty four (34) gumamela samples showed positive to the PCR amplification with begomovirus degenerate primers yielding an expected band size of ~1.5kb. Multiple sequence alignment of the obtained DNA-A fragments showed 97.0-99.0% similarities among isolates. Further sequence analysis of Philippine hibiscus leaf curl isolate with other hibiscus leaf curl viruses registered in the Genbank showed 98% nucleotide similarities with the Cotton leaf curl Multan virus.

Author Biographies

Lolita Martinez Dolores, Crop Science cluster, Institute of plant Breeding, University of the Philippines Los Banos (UPLB)

Institute of Plant Breeding, UPLB

Scientist 1

Maricel Gonzales, Crop Science Cluster, Institute of Plant Breeding, University of the Philippines Los Banos (UPLB)

Crop Science Cluster, Institute of plant Breeding, University of the philippines los Banos (UPLB)

University Research Associate

Pablito Magdalita, Crop Science Cluster, Institute of Plant Breeding, University of the Philippines Los Banos (UPLB)

Crop Science Cluster, Institute of plant Breeding, University of the philippines los Banos (UPLB)

Professor 1

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Published

2014-12-15

How to Cite

Dolores, L. M., Gonzales, M., & Magdalita, P. (2014). Isolation and Identification of a Begomovirus Associated With the Leaf Curl Disease of Gumamela (<i>Hibiscus rosa-sinensis</i>) in the Philippines. Asian Journal of Agriculture and Food Sciences, 2(6). Retrieved from https://ajouronline.com/index.php/AJAFS/article/view/2050

Issue

Vol. 2 No. 6 (2014): December 2014

Section

Articles

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