Profiles of Antibacterial Activities of Consecutive Extracts from Naked Oat against Several Food-Related Bacteria

Authors

  • Dong-Lin Hao
  • Qing-Ping Hu College of Life Sciences, Shanxi Normal University, 1 Gongyuan Street, Linfen,
  • Jiang-Lian Duan
  • Jian-Guo Xu

Keywords:

Naked oat, Extraction solvents, Consecutive extracts, Antibacterial activity

Abstract

The profiles of total phenolic content (TPC) and antibacterial activities of consecutive extracts from naked oat against several food-related bacteria were investigated in this paper. The results showed that the extracting solvent and fractional extraction significantly affected TPC, therefore influenced antibacterial activities of oat extracts. In our study, the ethyl acetate extracts from oats had the highest content of total polyphenols, followed by ethanol, chloroform, and water extracts, the lowest for petroleum ether extracts. No inhibitory effects of water extract on the tested bacteria was found, while other extracts from oats showed varying degrees of antibacterial activity against tested bacteria. Among these extracts, the ethyl acetate extracts had the highest antibacterial activities against the tested bacteria, followed by ethanol, chloroform, and petroleum ether extracts. These results indicated that selective extraction from natural sources, by an appropriate solvent or extraction method, is important for obtaining fractions with high antibacterial activity. In addition, though the exact mode of action of the ethyl acetate extracts on bacteria is still not clear, we concluded that one mechanism of action of extracts from oats against B. subtilis and S. dysenteriae was that ethyl acetate extracts disrupt the call wall based on SEM observations.

References

Alzoreky NS, Nakahara K. 2003. Antimicrobial activity of extracts from some edible plants commonly consumed in Asia. International Journal of Food Microbiology 80: 223–230.

Bahraminejad S, Asenstorfer RE, Riley IT, Schultz CJ. 2008. Analysis of the antimicrobial activity of flavonoids and saponins isolated from the shoots of oats (Avena sativa L.). Journal of Phytopathology 156: 1–7.

Burt S. 2004. Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology 94: 223–253.

Chen CY, Milbury PE, Kwak HK, Collins FW. 2004. Avenanthramides and phenolic acids from oats are bioavailable and act synergistically with vitamin C to enhance hamster and human LDL resistance to oxidation. Journal of Nutrition. 134: 1459–1466.

Cheok CY, Chin NL, Yusof YA, Law CL. 2012. Extraction of total phenolic content from Garcinia mangostana Linn. hull. I. Effects of solvents and UV–Vis spectrophotometer absorbance method. Food and Bioprocess Technology 5: 2928-2933.

Diao WR, Hu QP, Feng SS, Li WQ, Xu JG. 2013. Chemical composition and antibacterial activity of the essential oil from green huajiao (Zanthoxylum schinifolium) against selected foodborne pathogens. Journal of Agricultural and Food Chemistry 61: 6044−6049.

Dimberg LH, Molteberg EL, Solheim R, Frølich W. 1996. Variation in oat groats due to variety, storage and heat treatment. I: Phenolic compounds. Journal of Cereal Science 24: 263–272.

Emmons CL, Peterson DM. 2001. Antioxidant activity and phenolic content of oat as affected by cultivar and location. Crop Science 41: 1678–1681.

Emmons CL, Peterson DM, Paul GL. 1999. Antioxidant capacity of oat (Avena sativa L.) extracts. 2. in vitro antioxidant activity and contents of phenolic and tocol antioxidants. Journal of Agricultural and Food Chemistry 47: 4894–4898.

Jayaprakasha GK, Selvi T, Sakariah KK. 2003. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Research International 36: 117–122.

Joray MB, del Rollán MR, Ruiz GM, Palacios SM, Carpinella MC. 2011. Antibacterial activity of extracts from plants of central Argentina--Isolation of an active principle from Achyrocline satureioides. Planta Medica, 77: 95–100.

Miller GA, Youngs VL, Oplinger ES. 1980. Environmental and cultivar effects on oat phytic acid concentration. Cereal Chemistry 57: 189–191.

Moreau RA, Powell MJ, Hicks KB. 1996. Extraction and quantitative analysis of oil from commercial corn fiber. Journal of Agricultural and Food Chemistry 44: 2149–2154.

Negi PS, Chauhan AS, Sadia GA, Rohinishree YS, Ramteke RS. 2005. Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts. Food Chemistry 92: 119–124.

Petkov K, Biel W, Kowieska A, Jaskowska I. 2001. The composition and nutritive value of naked oat grain (Avena sativa var. nuda). Journal of Animal and Feed Sciences 10: 303–307.

Peterson DM. 2001. Oat antioxidants. Journal of Cereal Science 33: 115–129.

Peterson DM, Hahn MJ, Emmons CL. 2002. Oat avenanthramides exhibit antioxidant activities in vitro. Food Chemistry 79: 473–478.

Rebey IB, Bourgou S, Debez IBS, Karoui IJ, Sellami IH, Msaada K, Limam F, Marzouk B. 2012. Effects of extraction solvents and provenances on phenolic contents and antioxidant activities of cumin (Cuminum cyminum L.) seeds. Food and Bioprocess Technology 5: 2827–2836.

Rymbai H, Sharma RR, Srivasta M. 2011. Bio-colorants and its implications in health and food industry–a review. International Journal of Pharmacological Research 3: 2228–2244.

Shan B, Cai Y-Z, Brooks JD, Corke H. 2007. Antibacterial properties and major bioactive components of cinnamon stick (Cinnamomum burmannii): activity against foodborne pathogenic bacteria. Journal of Agricultural and Food Chemistry 55: 5484–5490.

Shin MS, Lee S, Lee KY, Lee HG. 2005. Structural and biological characterization of aminated-derivatized oat β-glucan. Journal of Agricultural and Food Chemistry 53: 5554-5558.

Sokmen A, Gulluce M, Akpulat HA, Daferera D, Tepe B, Polissiou M, Sokmen M, Sahin F. 2004. The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food Control 15: 627–634.

Sur R, Nigam A, Grote D, Liebel Frank, Southall MD. 2008. Avenanthramides, polyphenols from oats, exhibit anti-inflammatory and anti-itch activity. Archives of Dermatological Research 300: 569–574.

Sørensen HP, Madsen LS, Petersen J, Andersen JT, Hansen AM, Beck HC. 2010. Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase. Applied Biochemistry and Biotechnology 160: 1573–1584.

Tiwari BK, Valdramidis VP, Donnel CPO, Muthukumarappan K, Bourke P, Cullen PJ. 2009. Application of natural antimicrobials for food preservation. Journal of Agricultural and Food Chemistry 57: 5987–6000.

Turkmen N, Sari F, Sedat Velioglu Y. 2006. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin–Ciocalteu methods. Food Chemistry 99: 835–841.

Wang GL, Tang JH, Jiang D, Fang HJ, Liu ZB. 2006. Bacteriostatic action and mechanism of Sophora flavescens ait on Escherichia coli 01 C84010. Scientia Agricultura Sinica 39: 1018–1024.

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Published

2014-12-15

How to Cite

Hao, D.-L., Hu, Q.-P., Duan, J.-L., & Xu, J.-G. (2014). Profiles of Antibacterial Activities of Consecutive Extracts from Naked Oat against Several Food-Related Bacteria. Asian Journal of Agriculture and Food Sciences, 2(6). Retrieved from https://ajouronline.com/index.php/AJAFS/article/view/1841

Issue

Vol. 2 No. 6 (2014): December 2014

Section

Articles

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