Protein and Micronutrient Contents of <i>Moringa oleifera</i> (Murunga) Leaves Collected from Different Localities in Sri Lanka

Authors

  • R. Liyanage Institute of Fundamental Studies
  • C. Jayathilake Institute of Fundamental Studies
  • O. S. Perera Institute of Fundamental Studies
  • S. A. Kulasooriya Institute of Fundamental Studies
  • B. C. Jayawardana
  • S. Wimalasiri

Keywords:

Moringa oleifera, Micronutrients, Malnutrtion, Sri Lanka

Abstract

Deficiency in protein and micronutrients especially iron, zinc and calcium among children and pregnant mothers has become a major health issue in Sri Lanka today. Moringa oleifera is an important multipurpose tropical tree under-utilized for its nutritional and medicinal properties. In the present study, M. oleifera leaves collected from eight districts falling within different agro-climatic localities in Sri Lanka were analyzed for their protein and some micronutrient. The collected samples were dried, milled to particle sizes of less than 125 µm and protein as well as mineral contents were analyzed by Kjeldhal method and microwave assisted acid digestion followed by the Flame Atomic Absorption Spectrophotometer, respectively. Results revealed wide variations in the ranges of protein and micronutrients in Moringa leaves collected from the different localities, and among all the districts studied, leaves collected from Polonnaruwa contained significantly higher iron, zinc and protein contents compared to those from the other districts and calcium and magnesium were also present in considerable amounts. Therefore, Polonnaruwa district appears to offer the best environmental conditions for the intensive cultivation of moringa to obtain leaves of high nutritive value. However, moringa leaves collected from all the locations contain appreciable amounts of nutrients and could be used as a food supplement to combat protein and micronutrient malnutrition among the affected people of Sri Lanka.

 

Author Biographies

R. Liyanage, Institute of Fundamental Studies

Research Fellow

 

C. Jayathilake, Institute of Fundamental Studies

Research Assistant

Laboratory of Nutritional Biochemistry

 

O. S. Perera, Institute of Fundamental Studies

Research Assistant

Laboratory  of Nutritional Biochemistry

S. A. Kulasooriya, Institute of Fundamental Studies

Research Professor

References

“Energy and protein requirementsâ€, FAO Nutrition Meetings Report Series, No. 52; WHO Technical Report Series, No. 522, World Health Organization. 1973. (http://www.fao.org/docrep/003/aa040e/AA040E00.htm#TOC)

Potter NN and Hotchikiss JH, Food Science, (5th edition), CBS publishers and distributors, 1996.

Benoist B, McLean E, Egli E and Cogswell M, “Worldwide prevalence of anemia 1993-2005, Global Database on Anemia†World Health Organization, pp. 1-51, 2008. (http://whqlibdoc.who.int/publications/2008/9789241596657_ eng.pdf)

Brown KH, Peerson JM, Rivera L and Allen LH, “Effect of supplemental zinc on the growth and serum zinc concentrations of prepubertal children: A meta analysis of randomized controlled trialsâ€, American Journal of Clinical Nutrition, vol.75, pp.1062-1071, 2002. (http://ajcn.nutrition.org/content/75/6/ 1062.long)

Elmadfa I and Meyer AL, “Importance of food composition data to nutrition and public health. Europeanâ€, Journal of Clinical Nutrition, vol. 64, pp. S4–S7, 2010. (http://www.readcube.com/articles/10.1038/ejcn.2010.202)

Anwar F and Bhanger MI, “Analytical characterization of Moringa oleifera seed oil grown in temperate regions of Pakistanâ€, Journal of Agricultural and Food Chemistry, vol. 51, pp. 6558–6563, 2003. (http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/65/62)

Anderson J, Young L and Long E, “Potassium and Health. Food and Nutrition Seriesâ€, Fact Sheet No: 9, pp. 355, 2008. (www.michigan.gov/documents/mdch/Potassium_Fact_Sheet_3202917.pdf)

Food insecurity in the world: Economic crises-impacts and lessons learned, Rome, Food and Agriculture Organization of United Nations, 2009. (http://www.fao.org/docrep/012/i0876e/i0876e00.htm)

Iodine deficiency status of children in Sri Lanka: 2000-2001, Medical Research Institute, Department of Health Services, Colombo, Sri Lanka, 2001.

Child under-nutrition in Sri Lanka - a causal analysis, Medical Research Institute, Ministry of Healthcare and Nutrition, Colombo, 2006.

Census and Statistic reports Department of Census and statistics-Sri Lanka, 2002.

Amaranth S and Fernando K, “Widespread malnutrition among women and children†on Internet Technologies, http//: www.WSWS.org, 2012.

Hettiarachchi M Liyanage C Wickremasinghe R Hilmers DC and Abrams SA, “Prevalence and severity of micronutrient deficiency: a cross-sectional study among adolescents in Sri Lankaâ€, Asia Pacific Journal of Clinical Nutrition, vol. 15, pp.56-63, 2006. (file:///C:/Users/hp/Downloads/Hettiarachi.pdf)

Piyasena C and Mahamithawa AMASB, “Assessment of the anaemic status in Sri Lankaâ€, Medical Research Institute, Department of Health Services. 2003.

Price ML, "The Moringa Tree", Educational Concerns for Hunger Organization (ECHO) Technical Note. 1985, 2002. (http://chenetwork.org/files_pdf/Moringa.pdf)

Ramachandran C, Peter KV and Gopalakrishnan PK, “ Drumstick (Moringa oleifera): A multipurpose Indian Vegetableâ€, Econ Bot, vol. 34, pp. 27683, 1980. (http://www.jstor.org/discover/10.2307/4254186)

Dahot MU and Memon AR, “ Nutritive significance of oil extracted from Moringa oleifera seedsâ€, J Pharm Univer Karachi, vol.3, pp. 75 80, 1985.

Delaveau P and Boiteau P, “ Oils of Moringa oleifera and Moringa drouhardiiâ€, Plantes Medicinales et Phytotherapie, vol.14, pp.29-33, 1980. (http://eurekamag.com/research/006/021/006021276.php)

Dhar B and OP Gupta OP, “Nutritional value of Shigru (Moringa oleifera Lam.)†B.M.E.B.R, vol. 3, pp. 280-8, 1982. (http://stipulae.johnvanhulst.com/DOCS/PDF/Nutritional%20Value%20of%20Shigru.pdf)

U. Zongo, S. Zoungrana, A. Savadogo and A. Traoré, "Nutritional and Clinical Rehabilitation of Severely Malnourished Children with Moringa oleifera Lam. Leaf Powder in Ouagadougou (Burkina Faso)," Food and Nutrition Sciences, Vol. 4 No. 9, 2013, pp. 991-997. doi: 10.4236/fns.2013.49128.

M. C. P. Estrella, J. B. V. Mantaring, and G. Z. David, “A double blind randomised controlled trial on the use of malunggay (Moringa oleifera) for augmentation of the volume of breastmilk among non-nursing mothers of preterm infants,†The Philippine Journal of Pediatrics, vol. 49, pp. 3–6, 2000. (http://miracletrees.org/moringa-doc/moringa_breastfeeding_study.pdf)

Luqman S, Srivastava S, Kumar, R Maurya AK and Chanda D, “Experimental Assessment of Moringa oleifera Leaf and Fruit for Its Antistress, Antioxidant, and Scavenging Potential Using In Vitro and In Vivo Assaysâ€, Evidence-Based Complementary and Alternative Medicine, Vol. 2012, no. 519084, 2012. (http://www.ncbi.nlm.nih.gov/pubmed/22216055)

Iqbal S and Bhanger MI, “Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistanâ€, Journal of Food composition and Analysis, vol. 19, pp. 544-551, 2006. (http://www.sciencedirect.com/science/article/pii/S0889157505000670)

Coppin J A, “Study of the Nutritional and Medicinal values of Moringa oleifera leaves from Sub- Saharan Africa: Ghana, Rwanda Senegal and Zambiaâ€, MSc thesis, The State University of New Jersey, New Brunswick, New Jersey, 2008. (file:///C:/Users/hp/Downloads/rutgers-lib-24288-PDF-1%20(3). pdf)

Arabshahi DS, Devi DV and Urooj A, “Evaluation of antioxidant activity of some plant extracts and their heat, pH and storage stabilityâ€, Food Chemistry, vol. 100, pp. 1100-1105, 2007. (http://www.sciencedirect.com/science/article /pii/ S0308814605009830)

Fahey JW, “M. oleifera. A Review of the Medicinal Evidence for its Nutritional. Therapeutic and Prophylactic properties. Part 1†Tree for Life Journal, vol. 1, pp. 5, 2005. (http://www.tfljournal.org/article.php/20051201124931586)

Khawaja TM, Tahira M and Ikram UH, “Moringa oleifera : A natural gift- A reviewâ€, Journal of Pharmaceutical Science and Research, vol. 2, pp. 775- 781, 2010. (http://www.readperiodicals.com/201011/2280481861.html)

Nutrition for Health and Development: A global agenda for combating malnutrition, WHO/NHD/00.6, Geneva, 2000. (http://whqlibdoc.who.int/hq/2000/WHO_NHD_00.6.pdf)

Jongrungruangchok S, Bunrathep S and Songsak T, “Nutrients and minerals content of eleven different samples of Moringa oleifera cultivated in Thailandâ€, Journal of Health Research, vol. 24, pp.123-127, 2010. (http://herbs.ph/attachments/article/1301/)

Official Methods of Analysis, Association of Official Analytical Chemists, Washington, DC, USA. (15th edition) 1990.

Aslam M, Anver F, Nadeem R, Rashid U, Kazi TG and Nadeem M, “Mineral composition of Moringa oleifera leaves and pods from different regions of Punjab, Pakistanâ€, Asian Journal of Plant Sciences, vol.4, pp.417-421, 2005. (http://agris.fao.org/agris-search/search.do?recordID=DJ2012050175)

Yameogo CW Bengalayv MD Savagado A Nikiema PA and Traore SA, “Determination of Chemical Composition and Nutritive values of M.oleifera leavesâ€, Pakistan Journal of nutrition, vol. 10, pp. 264-268, 2011. (http://agris.fao.org/agris-search/search.do?recordID=DJ2012062859)

Elkhalifa AO, Ahmed SA and Adam S, “Nutritional evaluation of Moringa oleifera leaves and extracts’, Ahfad Journal, vol. 24, pp.113-22, 2007. (http://connection.ebscohost.com/c/articles/)

Fuglie LJ, “The miracle tree-the multiple attributes of Moringaâ€, Church World Service, Dakar, Senegal, 2001. (http://miracletrees.org/moringa-doc/Moringa_Presentation.pdf)

Moyo B, Masika PJ, Hugo A and Muchenje V, “Nutritional characterization of Moringa (Moringa oleifera Lam.) leavesâ€, African Journal of Biotechnology, vol.10, pp. 12925-12933, 2011. (http://www.ajol.info/index.php/ajb/article/ view/ 96497)

Oduro W, Ellis WO and Owusu D, “Nutritional potential of two leafy vegetables: Moringa oleifera and Ipomoea batatas leavesâ€, Scientific Research and Essay, vol.3, pp. 057-060, 2008. (http://eurekamag.com/research/032/ 600/032600053.php)

Ogbe AO and Affiku JP, “Proximate study, mineral and anti-nutrient composition of Moringa oleifera leaves harvested from Lafia, Nigeria: Potential benefits in poultry nutrition and healthâ€, Journal of Microbiology, Biotechnology and Food Sciences, vol.1, pp. 296-308, 2011. (http://en.engormix.com/MA-poultry-industry/nutrition/articles)

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Published

2014-08-13

How to Cite

Liyanage, R., Jayathilake, C., Perera, O. S., Kulasooriya, S. A., Jayawardana, B. C., & Wimalasiri, S. (2014). Protein and Micronutrient Contents of <i>Moringa oleifera</i> (Murunga) Leaves Collected from Different Localities in Sri Lanka. Asian Journal of Agriculture and Food Sciences, 2(4). Retrieved from https://ajouronline.com/index.php/AJAFS/article/view/1535

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