Isolation and Identification of Moulds Associated with Four Selected Snacks Sold in Nnamdi Azikiwe University, Awka and its Environs

Authors

  • J. E. Amadi Department of Botany, Nnamdi Azikiwe University, Awka, Anambra State PMB 5025, Awka
  • P. C. Onyejekwe
  • C. O. Ozokonkwo
  • M. O. Adebola

Keywords:

Food, Contamination, Snacks, Infestation, Moulds

Abstract

Food contamination is a major problem associated with food and snacks hawking in our society today. At a time when a lot of resources are being pumped into improving consumer health through food, it is ironic that more and more people are getting sick or dying from what they eat because of safety slips. This study was, therefore, carried out to determine the level of mould infestation associated with four selected snacks namely meat pie, buns, egg roll and doughnut. The snacks were purchased from vending sites in Amawbia, Aroma, Eke-Awka and Ifite  all within Awka, Anambra State. Nnamdi Azikiwe University is within Ifite. Results obtained showed that seven moulds species were associated with the selected snacks. The mould species isolated were Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Fusarium oxysporum, Fusarium solani, Penicillium digitatum and Rhizopus sp. Mean total fungal count from the four snacks was significantly higher in Meat pie (21.4 ± 2) and Egg roll (15.3±3) than in Doughnut (6.2 ± 2) and Buns (5.9 ± 1.6). Also, Eke Awka had mean total fungal count of 18.03 ± 3 which was significantly higher than at any other location. The lowest mean fungal count of 8.75 ± 1.8 was recorded at Amawbia. There was variation in the pattern of occurrence of the moulds in the snacks with P. digitatum having the highest percentage occurrence in all the locations put together.

References

• Annan-Prah, A., Amewowor, D. H. A. K., Osei-Kofi, J., Amoono, S. E., Akorli, E. Saka, S. Y. and Ndadi, H. A., “Street Foods: Handling, hygiene and client expectations in a World Heritage Site Town, Cape Coast, Ghanaâ€, African Journal of Microbiology Research 5(13): pp. 1629-1634, 2011.

• Barnette,H.L. and Hunter, B.B., Illustrated Genera of Imperfect Fungi; 4th edition Macmillian Publishing Company, New York, 1987.

• Chelea, M. and Jideani, I.A., “Incidence of Aflatoxin in some Ready to eat Foods sold in Market places in Bauchi Townâ€, Food Journal 17: pp. 51-65, 1996.

• Coda, R., Cassone, A., Rizzello, C. G., Nionelli, L., Cardinali, G., Gobbetti, M., “Antifungal activity of Wickerhamomyces anomalus and Lactobacillus plantarum during sour dough fermentation: identification of novel compounds and long-term effect during storage of wheat breadâ€, In Appl Environ Microbiol. 77(10):pp. 3484-3492, 2011.

• Culliney, K., Mold, expired dates and rat infestation prompt UK cake recall. www.bakeryand snacks.com/Regulation-Safety/ 2013.

• FAO, Street Foods Report of an FAO Technical Meeting on Street Foods, Calcutta India, 1995.

• Fawole, M.O. and Oso, B.A., Laboratory manual of Microbiology spectrum books Ltd Sunshine House Ibadan, Nigeria, 1988.

• Foskett, T.A., Barrett-Connor E.L., Wingard, D.L., “Practical Cookeryâ€, Snacks, light meals, Savouries and Convenience Foods.15:pp. 616-632, 2004.

• Gilbert, R.J., de Louvois, J., Donovan, T., Little, C., Nye, K., Ribeiro, C.D., Richards, J., Roberts, D. & Bolton, F.J., “Guidelines for the microbiological quality of some ready-to-eat foods sampled at the point of sale: A Working Group of the PHLS Advisory Committee for Food and Dairy Productsâ€, Communicable Disease and Public Health 3: pp. 163-167, 2000.

• James, A.S., “The role of breakfast in the treatment of obesity: A randomized clinical trialâ€, American Journal of Clinical Nutrition 55:pp. 645-651, 2005.

• James, M.J., Martins, J.L. and David, A.G., Modern Food Microbiology 782pp, 2005.

• Liyide, B. O., Frequency of Consumption of Local and Continental Snacks Amongst Secondary School Students in Abeokuta South Local Government Area of Ogun State, 2010.

• Mensah, P., Armar-Klemensu, A., Hammond, S., Haruna, A. and Nyarko, R., “Bacterial contaminants in lettuce, tomatoes, beef and goat meat from metropolitan Accraâ€, Ghana Med. Journal 35:pp. 1-6, 2001.

• Mensah, P., Yeboah-Manu, D., Owusu-Darko, K. & Ablordey, A., Street foods in Accra, Ghana: How safe are they? Bull. World Health Organisation. Volume 8: 7pp, 2002.

• Miller, A. A. and Ramsden, F., “Contamination of meat pies by salmonella in relation to baking and handling proceduresâ€, Journal of Applied Bacteriology, 18:pp. 565–580, 1955.

• Nielsen, A.C., “Ready to eat meals: Global highlights. A global consumer survey report. Safety Practice among Food Vendors in Ilorinâ€, J. Med. 5:pp. 120 – 124, 2006.

• Odu, N.N. and Akano, U.M., “The Microbiological Assessment of Ready-To-Eat-Food (Shawarma) In Port Harcourt City, Nigeriaâ€, Nature and Science 10(8):pp. 1-8, 2012.

• Ogundare, A. O. and Adetuyi, F. C., “Studies on the microbial population of bread baked with wheat flour from south western Nigeriaâ€, Food, Agriculture & Environment 1(3&4): pp. 85-87, 2003.

• Olumakaiye, M. F. and Ajayi, O.A., Food Consumption Patterns of Nigerian Adolescents and effect on body weight, 2008.

• Opeolu, B.O., Adebayo, K., Okuneye, P.A. and Badru, F.A., “Physiochemical and Microbial Assessment of Roadside Food and Water Sample in Lagos Environsâ€, J. Appl. Sci.Environ.14:pp. 29- 34, 2010.

• Oranusi, S., Omagbemi, F. and Eni, A.O., “Microbiological safety evaluation of snacks sold in fast food shops in Ota, Ogun State Nigeriaâ€, International journal of Agricultural and Food science 1(4):pp. 75-79, 2011.

• Phillips, M., “Analysis of Microbial Hazards related to time/temperature control of foods for safetyâ€, Comprehensive Review in Food Science and Food safety 2:pp. 33-35, 2003.

• Smith, J. P., Daifas, D.P., El-khoury, W., Koukoutsis, J. and El-khoury, A., “Shelf life and safety concerns of bakery products – A reviewâ€, In: Crit Rev Nutr. 44(1):pp. 19-55, 2004.

• Tsang, O., Guidelines for Ready-To-Eat Food. Road and Environmental Hygiene Department, Hong Kong, 2002.

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Published

2014-04-12

How to Cite

Amadi, J. E., Onyejekwe, P. C., Ozokonkwo, C. O., & Adebola, M. O. (2014). Isolation and Identification of Moulds Associated with Four Selected Snacks Sold in Nnamdi Azikiwe University, Awka and its Environs. Asian Journal of Agriculture and Food Sciences, 2(2). Retrieved from https://ajouronline.com/index.php/AJAFS/article/view/1079

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